Lipids by yarrowia lipolytica strains cultivated on glucose in batch cultures

[en] Oleaginous microorganisms, such as Yarrowia lipolytica, accumulate lipids that can have interesting applications in food biotechnology or the synthesis of biodiesel. Y. lipolytica yeast can have many advantages such as wide substrate range usage and robustness to extreme conditions, while under several culture conditions it can produce high lipid productivity. Based on this assumption, in this study, 12 different Yarrowia lipolytica strains were used to investigate microbial lipid production using a glucose-based medium under nitrogen-limited conditions in shake-flask cultivations. Twelve wild-type or mutant strains of Yarrowia lipolytica which were newly isolated or belonged to official culture collections were tested, and moderate lipid quantities (up to 1.30 g/L) were produced; in many instances, nitrogen limitation led to citric acid production in the medium. Lipids were mainly composed of C16 and C18 fatty acids. Most of the fatty acids of the microbial lipid were unsaturated and corresponded mainly to oleic, palmitic and linoleic acids. Linolenic acid (C18:3) was produced in significant quantities (between 10% and 20%, wt/wt of dry cell weight (DCW)) by strains H917 and Po1dL. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Disciplines :

Biotechnology

Author, co-author :

Carsanba, E.; Food Engineering Department, Faculty of Agriculture, Cukurova University, Adana, 01330, Turkey, Amyris Bioproducts Portugal, Unipessoal, Lda, Porto, 4169-005, Portugal

Papanikolaou, S.; Department of Food Science& Human Nutrition, Agricultural University of Athens, Athens, 11855, Greece

Fickers, Patrick ; Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies

Erten, H.; Food Engineering Department, Faculty of Agriculture, Cukurova University, Adana, 01330, Turkey

Language :

English

Title :

Lipids by yarrowia lipolytica strains cultivated on glucose in batch cultures

Publication date :

2020

Journal title :

Microorganisms

eISSN :

2076-2607

Publisher :

MDPI AG

Volume :

Issue :

Pages :

1-14

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

FDK-2014-3159

Available on ORBi :

since 18 February 2021

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