Article (Scientific journals)
Systematic study of liposomes composition towards efficient delivery of plasmid DNA as potential application of dermal fibroblasts targeting
Bellefroid, Coralie; Reusch, Céline; Lechanteur, Anna et al.
2021In International Journal of Pharmaceutics, 592, p. 120122
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Keywords :
flexible liposome; plasmid DNA; skin delivery; transfection; cytotoxicity
Abstract :
[en] The use of non-viral DNA vectors to topically treat skin diseases has demonstrated a high potential. However, vectors applied on the skin face extracellular barriers including the stratum corneum and intracellular barriers such as the endosomal escape and the nuclear targeting of the plasmid DNA. The aim of this study was to develop a formulation suitable for dermal application and effective for delivering plasmid DNA into cells. Different formulations were prepared using different cationic lipids (DOTAP, DC-Chol, DOTMA) and co-lipids (DOPE, DSPE). Lipoplexes were produced by complexing liposomes with plasmid DNA at different pDNA/CL (w/w) ratios. Our results showed that appropriate pDNA/CL ratios allowing total complexation of plasmid DNA differed depending on the structure of the lipid used. The transfection rates showed that (i) higher rates were obtained with DOTMA lipoplexes, (ii) DC-Chol lipoplexes provided a transfection twice as important as DOTAP lipoplexes and (iii) when DSPE was added, the cytotoxicity decreased while transfection rates were similar. We found that formulations composed of DC-Chol:DOPE:DSPE or DOTMA:DOPE were appropriate to complex plasmid DNA and to transfect human primary dermal fibroblasts with efficacy and limited cytotoxicity. Therefore, these formulations are highly promising in the context of gene therapy to treat skin diseases.
Research center :
CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Bellefroid, Coralie ;  Université de Liège - ULiège > CIRM
Reusch, Céline ;  Université de Liège - ULiège > Molecular Biology of Diseases-Gene Expression & Cancer
Lechanteur, Anna  ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique
Evrard, Brigitte  ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique
Debacq-Chainiaux
Mottet, Denis ;  Université de Liège - ULiège > Molecular Biology of Diseases-Gene Expression & Cancer
Piel, Géraldine ;  Université de Liège - ULiège > Département de pharmacie > Développement de nanomédicaments
Language :
English
Title :
Systematic study of liposomes composition towards efficient delivery of plasmid DNA as potential application of dermal fibroblasts targeting
Publication date :
2021
Journal title :
International Journal of Pharmaceutics
ISSN :
0378-5173
eISSN :
1873-3476
Publisher :
Elsevier, Netherlands
Volume :
592
Pages :
120122
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Skinano
Funders :
FEDER - Fonds Européen de Développement Régional [BE]
SPW DGO6 - Service Public de Wallonie. Economie, Emploi, Recherche [BE]
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since 24 November 2020

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