Systematic study of liposomes composition towards efficient delivery of plasmid DNA as potential application of dermal fibroblasts targeting

Bellefroid, Coralie; Reusch, Céline; Lechanteur, Anna; Evrard, Brigitte; Debacq-Chainiaux; Mottet, Denis; Piel, Géraldine

doi:10.1016/j.ijpharm.2020.120122

Article (Scientific journals)

Systematic study of liposomes composition towards efficient delivery of plasmid DNA as potential application of dermal fibroblasts targeting

Bellefroid, Coralie; Reusch, Céline; Lechanteur, Anna et al.

2021 • In International Journal of Pharmaceutics, 592, p. 120122

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/253024

DOI
10.1016/j.ijpharm.2020.120122

PubMed
33307161

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Keywords :

flexible liposome; plasmid DNA; skin delivery; transfection; cytotoxicity

Abstract :

[en] The use of non-viral DNA vectors to topically treat skin diseases has demonstrated a high potential. However, vectors applied on the skin face extracellular barriers including the stratum corneum and intracellular barriers such as the endosomal escape and the nuclear targeting of the plasmid DNA. The aim of this study was to develop a formulation suitable for dermal application and effective for delivering plasmid DNA into cells. Different formulations were prepared using different cationic lipids (DOTAP, DC-Chol, DOTMA) and co-lipids (DOPE, DSPE). Lipoplexes were produced by complexing liposomes with plasmid DNA at different pDNA/CL (w/w) ratios. Our results showed that appropriate pDNA/CL ratios allowing total complexation of plasmid DNA differed depending on the structure of the lipid used. The transfection rates showed that (i) higher rates were obtained with DOTMA lipoplexes, (ii) DC-Chol lipoplexes provided a transfection twice as important as DOTAP lipoplexes and (iii) when DSPE was added, the cytotoxicity decreased while transfection rates were similar. We found that formulations composed of DC-Chol:DOPE:DSPE or DOTMA:DOPE were appropriate to complex plasmid DNA and to transfect human primary dermal fibroblasts with efficacy and limited cytotoxicity. Therefore, these formulations are highly promising in the context of gene therapy to treat skin diseases.

Research Center/Unit :

CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège

Disciplines :

Pharmacy, pharmacology & toxicology

Author, co-author :

Bellefroid, Coralie ; Université de Liège - ULiège > CIRM

Reusch, Céline ; Université de Liège - ULiège > Molecular Biology of Diseases-Gene Expression & Cancer

Lechanteur, Anna ; Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique

Evrard, Brigitte ; Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique

Debacq-Chainiaux

Mottet, Denis ; Université de Liège - ULiège > Molecular Biology of Diseases-Gene Expression & Cancer

Piel, Géraldine ; Université de Liège - ULiège > Département de pharmacie > Développement de nanomédicaments

Language :

English

Title :

Systematic study of liposomes composition towards efficient delivery of plasmid DNA as potential application of dermal fibroblasts targeting

Publication date :

2021

Journal title :

International Journal of Pharmaceutics

ISSN :

0378-5173

eISSN :

1873-3476

Publisher :

Elsevier, Netherlands

Volume :

592

Pages :

120122

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

Skinano

Funders :

FEDER - Fonds Européen de Développement Régional
SPW DGO6 - Service Public de Wallonie. Economie, Emploi, Recherche

Available on ORBi :

since 24 November 2020

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