Article (Scientific journals)
Nosocomial outbreak caused by multidrug-resistant Pseudomonas aeruginosa producing IMP-13 metallo-β-lactamase
Pagani, L.; Colinon, C.; Migliavacca, R. et al.
2005In Journal of Clinical Microbiology, 43 (8), p. 3824-3828
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Keywords :
Italy; Pseudomonas aeruginosa; Cross Infection; Disease Outbreaks; Drug Resistance, Multiple, Bacterial; Humans; Pseudomonas Infections; Negibacteria
Abstract :
[en] An outbreak of Pseudomonas aeruginosa showing a multidrug-resistant (MDR) phenotype (including carbapenems, ceftazidime, cefepime, gentamicin, tobramycin, and fluoroquinolones) was observed, during a 5-month period, in a general intensive care unit of a large tertiary care and clinical research hospital in southern Italy. The outbreak involved 15 patients, with a total of 87 isolates, mostly from lower respiratory tract specimens. Analysis of isolates involved in the outbreak revealed production of metallo-β-lactamase (MBL) activity, and genotyping by pulsed-field gel electrophoresis of genomic DNA digested by SpeI revealed clonal relatedness among isolates. Molecular analysis of the MBL determinant showed the presence of a blaIMP-13 gene carried on a gene cassette inserted in a class 1 integron which also contained an aacA4 aminoglycoside resistance cassette encoding an AAC(6′)-Ib enzyme. The blaIMP-13-containing integron and its genetic environment appeared to be similar to those found in P. aeruginosa isolates producing IMP-13 from a hospital in Rome. The blaIMP-13 gene was not transferable by conjugation and was apparently carried on the chromosome. The outbreak was coincidental with a shortage of nursing personnel, and resolution was apparently associated with reinstatement of nursing personnel and reinforcement of general infection control practices within the intensive care unit. To our best knowledge this is the first description of a nosocomial outbreak of relatively large size caused by an IMF-producing gram-negative pathogen in Europe. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Disciplines :
Microbiology
Author, co-author :
Pagani, L.;  Dipartimento di Scienze Morfologiche Eidologiche e Cliniche, Sezione di Microbiologia, Università di Pavia, Pavia, Italy
Colinon, C.;  Dipartimento di Biologia Molecolare, Laboratorio di Fisiologia e Biotecnologia dei Microrganismi, Università di Siena, Siena, Italy
Migliavacca, R.;  Dipartimento di Scienze Morfologiche Eidologiche e Cliniche, Sezione di Microbiologia, Università di Pavia, Pavia, Italy
Labonia, M.;  IRCCS Casa Sollievo Della Sofferenza, San Giovanni Rotondo, Italy
Docquier, Jean-Denis ;  Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines
Nucleo, E.;  Dipartimento di Scienze Morfologiche Eidologiche e Cliniche, Sezione di Microbiologia, Università di Pavia, Pavia, Italy
Spalla, M.;  Servizio di Analisi Microbiologiche IRCCS S. Matteo, Pavia, Italy
Bergoli, M. L.;  IRCCS Casa Sollievo Della Sofferenza, San Giovanni Rotondo, Italy
Rossolini, G. M.;  Dipartimento di Biologia Molecolare, Laboratorio di Fisiologia e Biotecnologia dei Microrganismi, Università di Siena, Siena, Italy, Dipartimento di Biologia Molecolare, Università di Siena, Policlinico Santa Maria Alle Scotte, I-53100 Siena, Italy
Language :
English
Title :
Nosocomial outbreak caused by multidrug-resistant Pseudomonas aeruginosa producing IMP-13 metallo-β-lactamase
Publication date :
2005
Journal title :
Journal of Clinical Microbiology
ISSN :
0095-1137
eISSN :
1098-660X
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
Volume :
43
Issue :
8
Pages :
3824-3828
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 November 2020

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