Article (Scientific journals)
Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli
Lee, K.; Kim, C. K.; Yong, D. et al.
2010In Journal of Microbiological Methods, 83 (2), p. 149-152
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Keywords :
Carbapenemase screening; MacConkey agar; Modified Hodge test; Gram negative bacterium; Agar; Anti-Bacterial Agents; Bacterial Proteins; Culture Media; Gram-Negative Bacteria; Gram-Negative Bacterial Infections; Humans; Imipenem; Mass Screening; Microbial Sensitivity Tests; Bacilli (class); Negibacteria
Abstract :
[en] The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection. © 2010 Elsevier B.V.
Disciplines :
Microbiology
Author, co-author :
Lee, K.;  Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea
Kim, C. K.;  Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea
Yong, D.;  Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea
Jeong, S. H.;  Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea
Yum, J. H.;  Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea, Department of Clinical Laboratory Science, Dong-eui University, Busan, South Korea
Seo, Y. H.;  Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea
Docquier, Jean-Denis ;  Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines
Chong, Y.;  Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea
Language :
English
Title :
Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli
Publication date :
2010
Journal title :
Journal of Microbiological Methods
ISSN :
0167-7012
eISSN :
1872-8359
Publisher :
Elsevier, Netherlands
Volume :
83
Issue :
2
Pages :
149-152
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 November 2020

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