Atomic-Resolution Structure of a Class C β-Lactamase and Its Complex with Avibactam

X-ray diffraction; Aeromonas; Aeromonas enteropelogenes; Article; Pseudomonas aeruginosa; Gram negative infection; X ray crystallography; Amino Acid Sequence; Azabicyclo Compounds; Crystallography, X-Ray; Drug Design; Gram-Negative Bacterial Infections; Humans; Molecular Docking Simulation; Protein Conformation; Sequence Alignment

Abstract :

[en] β-Lactamases (BLs) are important antibiotic-resistance determinants that significantly compromise the efficacy of valuable β-lactam antibacterial drugs. Thus, combinations with BL inhibitor were developed. Avibactam is the first non-β-lactam BL inhibitor introduced into clinical practice. Ceftazidime–avibactam represents one of the few last-resort antibiotics available for the treatment of infections caused by near-pandrug-resistant bacteria. TRU-1 is a chromosomally encoded AmpC-type BL of Aeromonas enteropelogenes, related to the FOX-type BLs and constitutes a good model for class C BLs. TRU-1 crystals provided ultrahigh-resolution diffraction data for the native enzyme and for its complex with avibactam. A comparison of the native and avibactam-bound structures revealed new details in the conformations of residues relevant for substrate and/or inhibitor binding. Furthermore, a comparison of the TRU-1 and Pseudomonas aeruginosa AmpC avibactam-bound structures revealed two inhibitor conformations that were likely to correspond to two different states occurring during inhibitor carbamylation/recyclization. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

Disciplines :

Life sciences: Multidisciplinary, general & others
Biochemistry, biophysics & molecular biology
Microbiology

Author, co-author :

Pozzi, C.; Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Aldo Moro 2, Siena, 53100, Italy

Di Pisa, F.; Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Aldo Moro 2, Siena, 53100, Italy

De Luca, F.; Department of Medical Biotechnology, University of Siena, Via Aldo Moro 2, Siena, 53100, Italy

Benvenuti, M.; Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Aldo Moro 2, Siena, 53100, Italy

Docquier, Jean-Denis ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines

Mangani, S.; Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Aldo Moro 2, Siena, 53100, Italy

Language :

English

Title :

Atomic-Resolution Structure of a Class C β-Lactamase and Its Complex with Avibactam

Publication date :

2018

Journal title :

ChemMedChem

ISSN :

1860-7179

eISSN :

1860-7187

Publisher :

John Wiley and Sons Ltd

Volume :

Issue :

Pages :

1437-1446

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 13 November 2020

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