Biofilm mode of cultivation leads to an improvement of the entomotoxic patterns of two aspergillus species

Aspergillus flavus; Aspergillus oryzae; Biofilm; Biological control; Culex quinquefasciatus; Entomopathogenic microorganism; Mycotoxins; Secondary metabolites; Submerged culture

Abstract :

[en] Two fungi, i.e., Aspergillus flavus Link and Aspergillus oryzae (Ahlb.) E. Cohn, were cultivated according to two methodologies, namely submerged and biofilm cultures with the primary aim to use their secondary metabolites the supernatant CL50, and CL90 varied between 1.3% (v/v) to 12.7% (v/v) for incubation times from 24 to 72 h. While the A. flavus supernatant entomotoxicity was higher than this of A. oryzae, the biofilm culture application increased the efficiency of the former. Proteomic analysis of the supernatants revealed discrepancies among the two species and modes of cultivation. Furthermore, the secondary metabolite profiles of both Aspergillus cultures were verified. Aspergillic acid, beta-cyclopiazonic acid, cyclopiazonic acid, ferrineospergillin, flavacol, and spermadin A were most predominant. Generally, these secondary metabolites were present in higher concentrations in the supernatants of A. flavus and biofilm cultures. These molecular identifications correlated positively with entomotoxic activity. Noteworthy, the absence of carcinogenic aflatoxins was remarkable, and it will allow further valorization to produce A. flavus to develop potential biopesticides. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Disciplines :

Entomology & pest control

Author, co-author :

Francis, Frédéric ; Université de Liège - ULiège > Département GxABT > Gestion durable des bio-agresseurs

Druart, F.; Functional and Evolutionary Entomology, TERRA Research Center, Gembloux Agro-Bio Tech, University of Liege, Gembloux, 5030, Belgium, Microbial Processes and Interactions (MiPI), TERRA Research Center, Gembloux Agro-Bio Tech, University of Liege, Gembloux, 5030, Belgium

Di Mavungu, J. D.; Centre of Excellence in Mycotoxicology and Public Health, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, 9000, Belgium

De Boevre, M.; Centre of Excellence in Mycotoxicology and Public Health, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, 9000, Belgium

De Saeger, S.; Centre of Excellence in Mycotoxicology and Public Health, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, 9000, Belgium

Delvigne, Frank ; Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies

Language :

English

Title :

Biofilm mode of cultivation leads to an improvement of the entomotoxic patterns of two aspergillus species

Publication date :

2020

Journal title :

Microorganisms

eISSN :

2076-2607

Publisher :

MDPI AG

Volume :

Issue :

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

Herculesstichting: AUGE 13/13

Available on ORBi :

since 10 September 2020

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