Virus-Induced Gene Silencing (VIGS) in Cassava Using Geminivirus Agroclones.

[en] Virus-induced gene silencing (VIGS) is an efficient, low-cost, and rapid functional validation tool for candidate genes in planta. The VIGS approach is particularly suitable to perform reverse genetics studies in crop species. Here we present a detailed method to perform VIGS in cassava, from target gene fragment to agroinoculation and VIGS quantitation.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Syed-Shan-e-Ali, Zaïdi ; Université de Liège - ULiège > Département GxABT > Plant Sciences

Vasudevan, Kumar ; Université de Liège - ULiège > Département GxABT > Plant Sciences

Lentz, Ezequiel Matias

Vanderschuren, Hervé ; Université de Liège - ULiège > Département GxABT > Plant Sciences

Language :

English

Title :

Virus-Induced Gene Silencing (VIGS) in Cassava Using Geminivirus Agroclones.

Publication date :

2020

Journal title :

Methods in Molecular Biology

ISSN :

1064-3745

eISSN :

1940-6029

Volume :

2172

Pages :

51-64

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 09 July 2020

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