Optimization of the Impeller Design for Mesenchymal Stem Cell Culture on Microcarriers in Bioreactors

Bioreactor; Computational fluid dynamics (CFD); Impeller design; Multi-objective optimization; Particle suspension; Bioconversion; Bioreactors; Cell culture; Computational fluid dynamics; Energy dissipation; Impellers; Stem cells; Structural design; Suspensions (fluids); Computational fluid dynamics simulations; Energy dissipation function; Impeller geometry; Mesenchymal stem cell; Microcarriers; Particle suspensions; Relative sizes; Multiobjective optimization

Abstract :

[en] When agitating mesenchymal stem cells adhered on microcarriers in bioreactors, a compromise has to be found between sufficient particle suspension and limitation of hydromechanical stresses. The present study proposes a strategy to improve the design of an ‘elephant ear' impeller at the just-suspended state by varying its relative size, blade slope angle, and position in the reactor. To do that, computational fluid dynamics simulations were coupled with multi-objective optimization to minimize the hydromechanical stress encountered by the microcarriers. Two minimization criteria were considered: (P/V)@p and the energy dissipation function EDC. On the basis of 31 conditions, an optimal impeller geometry is proposed. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Disciplines :

Chemical engineering

Author, co-author :

Loubière, C.; Université de Lorraine, Laboratoire Réactions et Génie des Procédés, CNRS, LRGP, Nancy, 54000, France

Delafosse, Angélique ; Université de Liège - ULiège > Department of Chemical Engineering > Génie de la réaction et des réacteurs chimiques

Guedon, E.; Université de Lorraine, Laboratoire Réactions et Génie des Procédés, CNRS, LRGP, Nancy, 54000, France

Toye, Dominique ; Université de Liège - ULiège > Department of Chemical Engineering > Génie de la réaction et des réacteurs chimiques

Chevalot, I.; Université de Lorraine, Laboratoire Réactions et Génie des Procédés, CNRS, LRGP, Nancy, 54000, France

Olmos, E.; Université de Lorraine, Laboratoire Réactions et Génie des Procédés, CNRS, LRGP, Nancy, 54000, France

Language :

English

Title :

Optimization of the Impeller Design for Mesenchymal Stem Cell Culture on Microcarriers in Bioreactors

Publication date :

2019

Journal title :

Chemical Engineering and Technology

ISSN :

0930-7516

eISSN :

1521-4125

Publisher :

Wiley-VCH Verlag

Volume :

Issue :

Pages :

1702-1708

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

ANR - Agence Nationale de la Recherche [FR]
Interreg

Available on ORBi :

since 01 July 2020

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Bibliography

M. S. Croughan, J.-F. Hamel, D. I. Wang, Biotechnol. Bioeng. 1987, 29 (1), 130–141. DOI: https://doi.org/10.1002/bit.260290117
R. S. Cherry, E. T. Papoutsakis, Biotechnol. Bioeng. 1988, 32 (8), 1001–1014. DOI: https://doi.org/10.1002/bit.260320808
M.-L. Collignon, A. Delafosse, S. Calvo, C. Martin, A. Marc, D. Toye, E. Olmos, Biochem. Eng. J. 2016, 108, 30–43. DOI: https://doi.org/10.1016/j.bej.2015.10.020
G. Zhou, S. M. Kresta, AIChE J. 1996, 42 (9), 2476–2490. DOI: https://doi.org/10.1002/aic.690420908
S. Ibrahim, A. Nienow, Chem. Eng. Res. Des. 2004, 82 (9), 1082–1088. DOI: https://doi.org/10.1205/cerd.82.9.1082.44161
M.-L. Collignon, A. Delafosse, M. Crine, D. Toye, Chem. Eng. Sci. 2010, 65 (22), 5929–5941. DOI: https://doi.org/10.1016/j.ces.2010.08.027
V. Jossen, S. C. Kaiser, C. Schirmaier, J. Herrmann, A. Tappe, D. Eibl, A. Siehoff, C. van den Bos, R. Eibl, Pharm. Bioprocess. 2014, 2 (4), 311–322. DOI: https://doi.org/10.4155/PBP.14.29
A. Delafosse, M.-L. Collignon, A. Marc, D. Toye, E. Olmos, BMC Proc. 2015, 9 (9), 41. DOI: https://doi.org/10.1186/1753-6561-9-S9-P41
M. Chen, J. Wang, S. Zhao, C. Xu, L. Feng, Ind. Eng. Chem. Res. 2016, 55 (33), 9054–9063. DOI: https://doi.org/10.1021/acs.iecr.6b01660
N. Spogis, J. Nunhez, AIChE J. 2009, 55 (7), 1723–1735. DOI: https://doi.org/10.1002/aic.11804
C. Loubière, A. Delafosse, E. Guedon, I. Chevalot, D. Toye, E. Olmos, Chem. Eng. Sci. 2019, 203, 464–474. DOI: https://doi.org/10.1016/j.ces.2019.04.001
A. Delafosse, C. Loubière, S. Calvo, D. Toye, E. Olmos, Chem. Eng. Sci. 2018, 180, 52–63. DOI: https://doi.org/10.1016/j.ces.2018.01.001
S. C. Kaiser, V. Jossen, C. Schirmaier, D. Eibl, S. Brill, C. van den Bos, R. Eibl, Chem. Ing. Tech. 2013, 85, 95–102. DOI: https://doi.org/10.1002/cite.201200180
C. Schirmaier, V. Jossen, S. C. Kaiser, F. Jüngerkes, S. Brill, A. Safavi-Nab, A. Siehoff, C. van den Bos, D. Eibl, R. Eibl, Eng. Life Sci. 2014, 14, 292–303. DOI: https://doi.org/10.1002/elsc.201300134
P. Jüsten, G. Paul, A. Nienow, C. Thomas, Biotechnol. Bioeng. 1996, 52 (6), 672–684. DOI: https://doi.org/10.1002/(SICI)1097-0290(19961220)52:6<672::AID-BIT5>3.0.CO;2-L
A. W. Nienow, C. J. Hewitt, T. R. Heathman, V. A. Glyn, G. N. Fonte, M. P. Hanga, K. Coopman, Q. A. Rafiq, Biochem. Eng. J. 2016, 108, 24–29. DOI: https://doi.org/10.1016/j.bej.2015.08.003
T. R. Heathman, A. W. Nienow, Q. A. Rafiq, K. Coopman, B. Kara, C. J. Hewitt, Biochem. Eng. J. 2018, 136, 9–17. DOI: https://doi.org/10.1016/j.bej.2017.11.005
Y.-Y. Tsui, J.-R. Chou, Y.-C. Hu, J. Fluid Eng. 2006, 128 (4), 774–782. DOI: https://doi.org/10.1115/1.2201636
M. Roustan, Agitation. Mélange – Caractéristiques des mobiles d'agitation, Series in Techniques de l'Ingénieur, J3802, Saint-Denis 2005.
A. Delafosse, A. Line, J. Morchain, P. Guiraud, Chem. Eng. Res. Des. 2008, 86 (12), 1322–1330. DOI: https://doi.org/10.1016/j.cherd.2008.07.008
T. N. Zwietering, Chem. Eng. Sci. 1958, 8 (3/4), 244–253. DOI: https://doi.org/10.1016/0009-2509(58)85031-9
A. Nienow, Chem. Eng. Sci. 1968, 23 (12), 1453–1459. DOI: https://doi.org/10.1016/0009-2509(68)89055-4
S. Arvinth, P. S. Rao, T. Murugesan, Bioproc. Biosyst. Eng. 1996, 14 (2), 97–99. DOI: https://doi.org/10.1007/BF00387963