Article (Scientific journals)
Bovine Herpesvirus 1-Induced Apoptosis: Phenotypic Characterization of Susceptible Peripheral Blood Mononuclear Cells
Hanon, Emmanuel; Lambot, Michel; Hoornaert, Stephan et al.
1998In Archives of Virology, 143 (3), p. 441-52
Peer Reviewed verified by ORBi
 

Files


Full Text
2 OK Bovine herpesvirus.pdf
Author postprint (624.41 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] Bovine herpesvirus 1 (BHV-1), a member of the Alphaherpesvirinae, induces apoptotic cell death in peripheral blood mononuclear cells (PBMC). To investigate the process by which BHV-1 induces apoptosis, we determined the susceptibility of the three main PBMC subpopulations to BHV-1-induced apoptosis. This study shows that BHV-1 can induce apoptosis individually in T lymphocytes, B lymphocytes and monocytes. This conclusion is based on the following findings: (i) BHV-1 substantially reduces the percentages of viable T and B lymphocytes in PBMCs. (ii) Concomitant detection of cell phenotype and apoptosis indeed showed higher percentages of apoptotic T lymphocytes and B lymphocytes in BHV-1-infected PBMCs than in mock-infected cells. (iii) Each individual PBMC subpopulations (B lymphocytes, T lymphocytes and monocytes) undergo apoptosis when incubated with BHV-1. These data also suggest that BHV-1 does not require the recruitment of one or more individual PBMC subpopulations (e.g. cytotoxic cells) to induce apoptosis. Finally, we observed that BL-3 cells which have been characterized as bovine tumoral B lymphocytes also undergo apoptosis when incubated with BHV-1. Therefore, the use of the BL-3 cell line provides a new experimental model to investigate the apoptotic process induced by BHV-1 in vitro.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Hanon, Emmanuel ;  Université de Liège - ULiège > Faculty of Veterinary Medicine > Immunology/Vaccinology
Lambot, Michel ;  Université de Liège - ULiège > Faculty of Veterinary Medicine > Immunology/Vaccinology
Hoornaert, Stephan;  Université de Liège - ULiège > Faculty of Veterinary Medicine > Immunology/Vaccinology
Lyaku, Japhet;  Université de Liège - ULiège > Faculty of Veterinary Medicine > Immunology/Vaccinology
Pastoret, Paul-Pierre ;  Université de Liège - ULiège > Faculty of Veterinary Medicine > Immunology/Vaccinology
Language :
English
Title :
Bovine Herpesvirus 1-Induced Apoptosis: Phenotypic Characterization of Susceptible Peripheral Blood Mononuclear Cells
Publication date :
1998
Journal title :
Archives of Virology
ISSN :
0304-8608
eISSN :
1432-8798
Publisher :
Springer, Germany
Volume :
143
Issue :
3
Pages :
441-52
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 June 2020

Statistics


Number of views
46 (3 by ULiège)
Number of downloads
65 (2 by ULiège)

Scopus citations®
 
33
Scopus citations®
without self-citations
31
OpenCitations
 
30

Bibliography


Similar publications



Contact ORBi