Reference : Bovine Herpesvirus 1-Induced Apoptosis: Phenotypic Characterization of Susceptible Pe...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/249068
Bovine Herpesvirus 1-Induced Apoptosis: Phenotypic Characterization of Susceptible Peripheral Blood Mononuclear Cells
English
Hanon, Emmanuel [Université de Liège - ULiège > Faculty of Veterinary Medicine > Immunology/Vaccinology > >]
Lambot, Michel [Université de Liège - ULiège > Faculty of Veterinary Medicine > Immunology/Vaccinology > >]
Hoornaert, Stephan [Université de Liège - ULiège > Faculty of Veterinary Medicine > Immunology/Vaccinology > >]
Lyaku, Japhet [Université de Liège - ULiège > Faculty of Veterinary Medicine > Immunology/Vaccinology > >]
Pastoret, Paul-Pierre mailto [Université de Liège - ULiège > Faculty of Veterinary Medicine > Immunology/Vaccinology > >]
1998
Archives of Virology
Springer
143
3
441-52
Yes (verified by ORBi)
International
0304-8608
1432-8798
Germany
[en] Bovine herpesvirus 1 (BHV-1), a member of the Alphaherpesvirinae, induces apoptotic cell death in peripheral blood mononuclear cells (PBMC). To investigate the process by which BHV-1 induces apoptosis, we determined the susceptibility of the three main PBMC subpopulations to BHV-1-induced apoptosis. This study shows that BHV-1 can induce apoptosis individually in T lymphocytes, B lymphocytes and monocytes. This conclusion is based on the following findings: (i) BHV-1 substantially reduces the percentages of viable T and B lymphocytes in PBMCs. (ii) Concomitant detection of cell phenotype and apoptosis indeed showed higher percentages of apoptotic T lymphocytes and B lymphocytes in BHV-1-infected PBMCs than in mock-infected cells. (iii) Each individual PBMC subpopulations (B lymphocytes, T lymphocytes and monocytes) undergo apoptosis when incubated with BHV-1. These data also suggest that BHV-1 does not require the recruitment of one or more individual PBMC subpopulations (e.g. cytotoxic cells) to induce apoptosis. Finally, we observed that BL-3 cells which have been characterized as bovine tumoral B lymphocytes also undergo apoptosis when incubated with BHV-1. Therefore, the use of the BL-3 cell line provides a new experimental model to investigate the apoptotic process induced by BHV-1 in vitro.
http://hdl.handle.net/2268/249068
10.1007/s007050050301

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