Article (Scientific journals)
The two copies of the zinc and cadmium ZIP6 transporter of Arabidopsis halleri have distinct effects on cadmium tolerance.
Spielmann, Julien; Ahmadi, Hassan; Scheepers, Maxime et al.
2020In Plant, cell & environment
Peer reviewed
 

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Keywords :
Arabidopsis halleri; cadmium tolerance; gene duplication; hyperaccumulation; male sterility; sub-functionalization; zinc
Abstract :
[en] Plants have the ability to colonize highly diverse environments. The zinc and cadmium hyperaccumulator Arabidopsis halleri has adapted to establish populations on soils covering an extreme range of metal availabilities. The A. halleri ZIP6 gene presents several hallmarks of hyperaccumulation candidate genes: it is constitutively highly expressed in roots and shoots and is associated with a zinc accumulation quantitative trait locus. Here, we show that AhZIP6 is duplicated in the A. halleri genome. The two copies are expressed mainly in the vasculature in both A. halleri and A. thaliana, indicative of conserved cis regulation, and acquired partial organ specialization. Yeast complementation assays determined that AhZIP6 is a zinc and cadmium transporter. AhZIP6 silencing in A. halleri or expression in A. thaliana alters cadmium tolerance, but has no impact on zinc and cadmium accumulation. AhZIP6-silenced plants display reduced cadmium uptake upon short-term exposure, adding AhZIP6 to the limited number of Cd transporters supported by in planta evidence. Altogether, our data suggest that AhZIP6 is key to fine-tune metal homeostasis in specific cell-types. This study additionally highlights the distinct fates of duplicated genes in A. halleri. This article is protected by copyright. All rights reserved.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Spielmann, Julien ;  Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Ahmadi, Hassan
Scheepers, Maxime ;  Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Weber, Michael
Nitsche, Sarah
Carnol, Monique  ;  Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Ecologie végétale et microbienne
Bosman, Bernard ;  Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Ecologie végétale et microbienne
Kroymann, Juergen
Motte, Patrick ;  Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Clemens, Stephan
Hanikenne, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Language :
English
Title :
The two copies of the zinc and cadmium ZIP6 transporter of Arabidopsis halleri have distinct effects on cadmium tolerance.
Publication date :
2020
Journal title :
Plant, cell & environment
ISSN :
0140-7791
eISSN :
1365-3040
Peer reviewed :
Peer reviewed
Commentary :
This article is protected by copyright. All rights reserved.
Available on ORBi :
since 24 June 2020

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