Jug r 6 is the allergenic vicilin present in walnut responsible for IgE cross-reactivities to other tree nuts and seeds

[en] Walnuts are ranked high in the list of the culprit foods inducing severe allergic reactions. Jug r 2 has been identified as a major allergen in common walnut by cDNA cloning from a somatic cell line. So far, studies were performed on the allergenic activity of recombinant Jug r 2, yet there is still no evidence about the physicochemical characteristics of the natural allergen. Therefore, we aimed to purify and deeply characterize natural Jug r 2 and to assess IgE cross-reactivity among vicilins from different tree nuts. Extensive mass spectrometry analysis of the obtained purified vicilin allowed identification of the protein sequence that displayed only 44% identity to Jug r 2. The newly identified vicilin (Jug r 6) was recognized by IgE of 26% in walnut allergic patients’ sera tested. In contrast to Jug r 2, Jug r 6 displayed a remarkable level of cross-reactivity when tested with homologues from hazelnut, sesame and pistachio. It is the first report showing the necessity of proteomic studies to improve allergy component resolved diagnosis. © 2018, The Author(s).

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Dubiela, Pawel; Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria

Kabasser, Stefan; Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria

Smargiasso, Nicolas ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)

Geiselhart, Sabine; Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria

Bublin, Merima; Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria

Hafner, Christine; Department of Dermatology, University Hospital St. Poelten, Karl Landsteiner University of Health Sciences, St. Poelten, Austria

Mazzucchelli, Gabriel ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)

Hoffmann-Sommergruber, Karin; Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria

Language :

English

Title :

Jug r 6 is the allergenic vicilin present in walnut responsible for IgE cross-reactivities to other tree nuts and seeds

Publication date :

2018

Journal title :

Scientific Reports

eISSN :

2045-2322

Publisher :

Nature Publishing Group, London, United Kingdom

Volume :

Issue :

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 23 May 2020

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