Engineering Yarrowia lipolytica for the Synthesis of Glutathione from Organic By-Products

[en] Tripeptide glutathione, which plays important roles in many cellular mechanisms, is also a biotechnology-oriented molecule with applications in medicine, food and cosmetic. Here, the engineering of the yeast Yarrowia lipolytica for the production of this metabolite at high titer values from various agro-industrial by-products is reported. The constitutive overexpression of the glutathione biosynthetic genes GSH1 and GSH2 encoding respectively γ-glutamylcysteine synthetase and glutathione synthetase, together with the INU1 gene from Kluyveromyces marxianus encoding inulinase yielded a glutathione titer value and a productivity of 644 nmol/mg protein and 510 µmol/gDCW, respectively. These values were obtained during bioreactor batch cultures in a medium exclusively comprising an extract of Jerusalem artichoke tuber, used as a source of inulin, and ammonium sulfate, used as a nitrogen source.

Research center :

MiPI-Gembloux Agrobiotech

Disciplines :

Biotechnology

Author, co-author :

Do Thi Hoang, Diem ; Université de Liège - ULiège > Terra

Fickers, Patrick ; Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies

Language :

English

Title :

Engineering Yarrowia lipolytica for the Synthesis of Glutathione from Organic By-Products

Publication date :

23 April 2020

Journal title :

Microorganisms

eISSN :

2076-2607

Publisher :

Molecular Diversity Preservation International (MDPI), Basel, Switzerland

Special issue title :

Yeast and Fungal Metabolites

Volume :

Issue :

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

VIED, grant N° 4278/QD-BGDDT

Funders :

Vietnam International Education Development

Available on ORBi :

since 29 April 2020

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