Raman imaging as a new analytical tool for the quality control of the monitoring of osteogenic differentiation in forming 3D bone tissue

De Bleye, Charlotte; Fontaine, Marie; Dumont, Elodie; Sacre, Pierre-Yves; Hubert, Philippe; Theys, Nicolas; Ziemons, Eric

doi:10.1016/j.jpba.2020.113319

Article (Scientific journals)

Raman imaging as a new analytical tool for the quality control of the monitoring of osteogenic differentiation in forming 3D bone tissue

De Bleye, Charlotte; Fontaine, Marie; Dumont, Elodie et al.

2020 • In Journal of Pharmaceutical and Biomedical Analysis, 186, p. 113319

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/246934

DOI
10.1016/j.jpba.2020.113319

PubMed
32361470

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Keywords :

Raman imaging; Mineral to matrix ratio determination; Osteogenic differentiation monitoring; Method reliability study; Quality control

Abstract :

[en] In this study, adipose-derived stem cells (ASCs) are used to produce 3D bone grafts. The safety and the feasibility of using these bone grafts have been already showed and quality controls are already implemented. However, a cheaper, fast and non-destructive technique is required to monitor the osteogenic differentiation process. Here, the use of Raman imaging to monitor the synthesis of the extracellular matrix and its progressive mineralization occurring during the osteogenic differentiation process is investigated for the first time on a 3D in forming bone tissue. The attention was focused on Raman bands related to this matrix belonging to phosphate, phenylalanine and hydroxyproline, which are very distinctive and intense. The kinetic of the osteogenic differentiation process was first compared between a 2D and a 3D forming bone tissue. It was observed that the kinetics of the osteogenic differentiation process is slower in 3D in forming bone tissue. In a second step, an evaluation of the reliability of the Raman imaging method was performed including a study of the influence of the harvest biopsies position on the forming 3D bone tissue. The repeatability and the specificity of this method were also demonstrated. In a last step, several batches of ASCs were cultured and analyzed in 3D at different time points using Raman imaging. From the mean Raman spectra, mineral to matrix ratios (MTMR) were determined and used to evaluate the formation of mineral deposits accompanying the extracellular matrix synthesis which is indicative of an ongoing osteogenic differentiation process. These ratios peaked between the day 35 and 49. This observation was very interesting since it corresponds to the time at which the 3D bone grafts are used for the patient surgery. To conclude, Raman imaging allowed fast acquisition and time-resolved monitoring in vitro of the mineralization of extracellular matrix during osteogenic differentiation.

Research Center/Unit :

CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège

Disciplines :

Pharmacy, pharmacology & toxicology

Author, co-author :

De Bleye, Charlotte ; Université de Liège - ULiège > Département de pharmacie > Département de pharmacie

Fontaine, Marie; Novadip Biosciences

Dumont, Elodie ; Université de Liège - ULiège > Département de pharmacie > Chimie analytique

Sacre, Pierre-Yves ; Université de Liège - ULiège > Département de pharmacie > Chimie analytique

Hubert, Philippe ; Université de Liège - ULiège > Département de pharmacie > Chimie analytique

Theys, Nicolas; Novadip Biosciences

Ziemons, Eric ; Université de Liège - ULiège > Département de pharmacie > Chimie analytique

Language :

English

Title :

Raman imaging as a new analytical tool for the quality control of the monitoring of osteogenic differentiation in forming 3D bone tissue

Publication date :

15 April 2020

Journal title :

Journal of Pharmaceutical and Biomedical Analysis

ISSN :

0731-7085

eISSN :

1873-264X

Publisher :

Elsevier, Amsterdam, Netherlands

Volume :

186

Pages :

113319

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

DGTRE - Région wallonne. Direction générale des Technologies, de la Recherche et de l'Énergie

Available on ORBi :

since 27 April 2020

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