[en] Within the family of serotonin (5-HT) receptors, the 5-HT1A subtype is particularly interesting as it may be involved in various physiological processes or psychological disorders. The p-[18F]MPPF, a highly selec- tive 5-HT1A antagonist, is used for in vivo studies in human or animal by means of positron emission tomography (PET) [1].
In order to selectively extract p-[18 F]MPPF and its main metabolites from plasma, molecularly imprinted polymer (MIP) was prepared against these compounds by using the p-MPPF as template. For the control of the selectivity, non-imprinted polymer (NIP) was also synthesized without template. The MIP sorbent, packed in disposable extraction cartridges (DECs), was then evaluated as molecularly imprinted solid- phase extraction (MISPE) prior to the LC determination. The conditions of extraction were evaluated in order to obtain the highest selective retention of the p-[18F]MPPF and its metabolites on this MIP. The MIP selectivity was exploited in the loading and washing steps by adjusting the pH of plasma samples at a suitable value and by selecting mixtures for the washing step to limit the contribution of non- specific interactions. Other important parameters involved in the conditioning and elution steps were also studied. Finally, a pre-validation was carried out with optimal extraction conditions to demonstrate the performance of this MISPE-LC method as a generic method in the context of evaluation of new MISPE for p-[18F]MPPF and its potential for metabolites extraction from human plasma.
Research Center/Unit :
CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège
Disciplines :
Pharmacy, pharmacology & toxicology Chemistry
Author, co-author :
Lecomte, Frederic ✱; Université de Liège - ULiège > Département de Pharmacie > Chimie Analytique > Doct. sc. bioméd. & pharma. (Bologne)
Aerts, Joël ✱; Université de Liège - ULiège > CRC In vivo Imaging-Preclinical Imaging
Plenevaux, Alain ; Université de Liège - ULiège > CRC In vivo Imaging-Preclinical Imaging
Defraiteur, Caroline ; Université de Liège - ULiège > CRC In vivo Imaging-Preclinical Imaging
Chappuis-Hugon, Florence; ESPCI Paris, PSL University, > Dept of Analytical, Bioanalytical Sciences and Miniaturization
Rozet, Eric; Pharmalex
CHIAP, Patrice ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Unité GLP-AEPT
Luxen, André ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de chimie organique de synthèse
Pichon, Valérie; ESPCI Paris, PSL University > Dept of Analytical, Bioanalytical Sciences and Miniaturization
Hubert, Philippe ✱; Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Hubert, Cédric ✱; Université de Liège - ULiège > Département de pharmacie > Chimie analytique
✱ These authors have contributed equally to this work.
Language :
English
Title :
Performance evaluation of a MIP for the MISPE-LC determination of p-[18F]MPPF and a potential metabolite in human plasma
Publication date :
20 February 2020
Journal title :
Journal of Pharmaceutical and Biomedical Analysis
ISSN :
0731-7085
eISSN :
1873-264X
Publisher :
Elsevier
Volume :
180
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
FP6 STRP516984 MI-lab- on-chip
Funders :
Fonds Léon Fredericq F.R.S.-FNRS - Fonds de la Recherche Scientifique
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