Differential production of cytokines and activation of NF-kappa B in HPV-transformed keratinocytes

[en] We have proposed that chronic infection of keratinocytes by HPV modifies the expression of potentially important cytokines by interfering with the NF-kappaB signal pathway We evaluated the constitutive and IL-1beta-induced expression of GM-CSF and TNF-alpha and the expression/activity of NF-kappaB in HPV+ and HPV- cell lines. Despite the enhanced expression of the functional components of the NF-kappaB signaling pathway in HPV+ cell lines by a mechanism implicating the HPV oncoprotein E6, the constitutive activity of NF-kappaB and the expression of GM-CSF/TNF-alpha were significantly reduced relative to the HPV- cell line and normal keratinocytes. In contrast, we observed a superactivation of NF-kappaB activity after IL-1beta stimulation, a strong and transient induction of GM-CSF/TNF-alpha mRNA, but undetectable levels of secreted proteins in HPV+ cell lines. Our data demonstrate that E6 modulates the NF-kappaB signaling pathway and suggest that other HPV proteins also interfere with GM-CSF/TNF-alpha expression by transcriptional and/or posttranscriptional mechanisms. (C) 2002 Elsevier Science (USA).

Disciplines :

Oncology
Immunology & infectious disease

Author, co-author :

Havard, L.

Delvenne, Philippe ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques

Frare, P.

Boniver, Jacques ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques

Giannini, S. L.

Language :

English

Title :

Differential production of cytokines and activation of NF-kappa B in HPV-transformed keratinocytes

Publication date :

05 July 2002

Journal title :

Virology

ISSN :

0042-6822

eISSN :

1096-0341

Publisher :

Academic Press Inc Elsevier Science, San Diego, United States - California

Volume :

298

Issue :

Pages :

271-285

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 29 September 2009

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