Article (Scientific journals)
Investigation of the effect of different extracellular factors on the lipase production by Yarrowia lipolityca on the basis of a scale-down approach
Kar, Tambi; Delvigne, Frank; Masson et al.
2008In Journal of Industrial Microbiology and Biotechnology, 35 (9), p. 1053-1059
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Keywords :
methyl oleat; heterogeneities; scale-up; LIP2 expression
Abstract :
[en] The influence of three extracellular factors (namely, the methyl oleate dispersion in the broth, the dissolved oxygen variations, and the pH fluctuation) on the lipase production by Y. lipolytica in batch bioreactor has been investigated in different scale-down apparatus. These systems allow to reproduce the hydrodynamic phenomena encountered in large-scale equipments for the three specified factors. The effects of the extracellular factors have been observed at three distinct levels: the microbial growth, the extracellular lipase production, and the induction of the gene LIP2 encoding for the main lipase of Y. lipolytica. Among the set of environmental factors investigated, the dissolved oxygen fluctuations generated in a controlled scale-down reactor (C-SDR) have led to the more pronounced physiological effect by decreasing the LIP2 gene expression level. The other environmental factors observed in a partitioned scale-down reactor, i.e., the methyl oleate dispersion and the pH fluctuations, have led to a less severe stress traduced only by a decrease of the microbial yield and thus of the extracellular lipase specific production rate.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Kar, Tambi ;  Université de Liège - ULiège > Doct. sc. agro. & ingé. biol.
Delvigne, Frank  ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Masson
Destain, Jacqueline ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Thonart, Philippe ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles
Language :
English
Title :
Investigation of the effect of different extracellular factors on the lipase production by Yarrowia lipolityca on the basis of a scale-down approach
Publication date :
10 September 2008
Journal title :
Journal of Industrial Microbiology and Biotechnology
ISSN :
1367-5435
eISSN :
1476-5535
Publisher :
SPRINGER, D-69121 HEIDELBERG, Germany
Volume :
35
Issue :
9
Pages :
1053-1059
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 29 September 2009

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