Article (Scientific journals)
Limited cross reactivity among arginine kinase allergens from mealworm and cricket edible insects
Francis, Frédéric; Doyen, V.; Debaugnies, F. et al.
2019In Food Chemistry, 276, p. 714-718
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Keywords :
Arginine kinase; Insect allergen; Proteomics; Purification; Western blot; Amino acids; Arginine; Enzymes; Molecular biology; Allergic reactions; Cross reactivity; European Countries; Immunoglobulin E; Tenebrio molitor; Western blots; Allergens
Abstract :
[en] Insects are seen as a solution to the increasing demand for protein sources for food. However, entomophagy has unfortunately been linked to allergic reactions in Europe with people with professional contacts. As mealworms (Tenebrio molitor) and crickets (Acheta domesticus) have recently become commercially available (both whole or in food formulation) in several European countries, this research assessed the cross allergenicity of arginine kinase (AK). Based on the collection of sera from a entomology laboratory staff, oven cooked insects but also purified AK fractions were tested. Immunoblotting against the protein extracts revealed different Immunoglobulin E reactivity of sera according to the insect target species: two bands (40 and 14 kDa) for crickets and a pattern including light responses at 17, 25 and 37 kDa for mealworms. Focusing on AK, low specific allergenicity was here illustrated and discussed in relation to the development of a safe edible insect consumption by humans. © 2018
Disciplines :
Entomology & pest control
Author, co-author :
Francis, Frédéric  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Gestion durable des bio-agresseurs
Doyen, V.;  CHU Brugman, Immunology IRIS Laboratory, Belgium
Debaugnies, F.;  CHU Brugman, Immunology IRIS Laboratory, Belgium
Mazzucchelli, Gabriel  ;  Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Caparros Megido, Rudy  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Gestion durable des bio-agresseurs
Alabi, Taofic ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Gestion durable des bio-agresseurs
Blecker, Christophe ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > SMARTECH
Haubruge, Eric  ;  Université de Liège - ULiège > Gestion durable des bio-agresseurs
Corazza, F.;  CHU Brugman, Immunology IRIS Laboratory, Belgium
Language :
English
Title :
Limited cross reactivity among arginine kinase allergens from mealworm and cricket edible insects
Publication date :
2019
Journal title :
Food Chemistry
ISSN :
0308-8146
eISSN :
1873-7072
Publisher :
Elsevier Ltd
Volume :
276
Pages :
714-718
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 13 December 2019

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