Limited cross reactivity among arginine kinase allergens from mealworm and cricket edible insects

Arginine kinase; Insect allergen; Proteomics; Purification; Western blot; Amino acids; Arginine; Enzymes; Molecular biology; Allergic reactions; Cross reactivity; European Countries; Immunoglobulin E; Tenebrio molitor; Western blots; Allergens

Abstract :

[en] Insects are seen as a solution to the increasing demand for protein sources for food. However, entomophagy has unfortunately been linked to allergic reactions in Europe with people with professional contacts. As mealworms (Tenebrio molitor) and crickets (Acheta domesticus) have recently become commercially available (both whole or in food formulation) in several European countries, this research assessed the cross allergenicity of arginine kinase (AK). Based on the collection of sera from a entomology laboratory staff, oven cooked insects but also purified AK fractions were tested. Immunoblotting against the protein extracts revealed different Immunoglobulin E reactivity of sera according to the insect target species: two bands (40 and 14 kDa) for crickets and a pattern including light responses at 17, 25 and 37 kDa for mealworms. Focusing on AK, low specific allergenicity was here illustrated and discussed in relation to the development of a safe edible insect consumption by humans. © 2018

Disciplines :

Entomology & pest control

Author, co-author :

Francis, Frédéric ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Gestion durable des bio-agresseurs

Doyen, V.; CHU Brugman, Immunology IRIS Laboratory, Belgium

Debaugnies, F.; CHU Brugman, Immunology IRIS Laboratory, Belgium

Mazzucchelli, Gabriel ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)

Caparros Megido, Rudy ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Gestion durable des bio-agresseurs

Alabi, Taofic ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Gestion durable des bio-agresseurs

Blecker, Christophe ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > SMARTECH

Haubruge, Eric ; Université de Liège - ULiège > Gestion durable des bio-agresseurs

Corazza, F.; CHU Brugman, Immunology IRIS Laboratory, Belgium

Language :

English

Title :

Limited cross reactivity among arginine kinase allergens from mealworm and cricket edible insects

Publication date :

2019

Journal title :

Food Chemistry

ISSN :

0308-8146

eISSN :

1873-7072

Publisher :

Elsevier Ltd

Volume :

276

Pages :

714-718

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 13 December 2019

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