Characterization of the proteins encoded by the Bacillus subtilis yoxA-dacC operon.

[en] Abstract In Bacillus subtilis, the yoxA and dacC genes were proposed to form an operon. The yoxA gene was overexpressed in Escherichia coli and its product fused to a polyhistidine tag was purified. An aldose-1-epimerase or mutarotase activity was measured with the YoxA protein that we propose to rename as GalM by analogy with its counterpart in E. coli. The peptide d-Glu-delta-m-A(2)pm-d-Ala-m-A(2)pm-d-Ala mimicking the B. subtilis and E. coli interpeptide bridge was synthesized and incubated with the purified dacC product, the PBP4a. A clear dd-endopeptidase activity was obtained with this penicillin-binding protein, or PBP. The possible role of this class of PBP, present in almost all bacteria, is discussed.

Research center :

CIP - Centre d'Ingénierie des Protéines - ULiège
GIGA CRC (Cyclotron Research Center) In vivo Imaging-Aging & Memory - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Duez, Colette ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Zervosen, Astrid ; Université de Liège - ULiège > Centre de recherches du cyclotron

Teller, Nathalie; Université de Liège - ULiège > Centre de recherche du cyclotron

Melkonian, Rémy; Université de Liège - ULiège > Centre d'ingénierie des protéines

Banzubazé, Emmanuel

Bouillenne, Fabrice ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Luxen, André ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie organique de synthèse - Centre de recherches du cyclotron

Frère, Jean-Marie ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Language :

English

Title :

Characterization of the proteins encoded by the Bacillus subtilis yoxA-dacC operon.

Alternative titles :

[fr] Caractérisation des protéines codées par l'opéron yoxA-dacC de Bacillus subtilis

Publication date :

2009

Journal title :

FEMS Microbiology Letters

ISSN :

0378-1097

eISSN :

1574-6968

Publisher :

Blackwell Publishing, Oxford, United Kingdom

Volume :

300

Pages :

42-47

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

PAI, ARC et EUR-INTAFAR

Available on ORBi :

since 28 September 2009

Statistics

Number of views

104 (29 by ULiège)

Number of downloads

6 (6 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

Bibliography

Bouffard GG, Rudd KE Adhya SL (1994) Dependence of lactose metabolism upon mutarotase encoded in the gal operon in Escherichia coli. J Mol Biol 244 : 269 278.
Duez C, Vanhove M, Gallet X, Bouillenne F, Docquier J-D, Brans A Frère J-M (2001) Purification and characterization of PBP4a, a new low-molecular-weight Penicillin-Binding Protein from Bacillus subtilis. J Bacteriol 183 : 1595 1599.
Frey PA (1996) The Leloir pathway: a mechanistic imperative for three enzymes to change the stereochemical configuration of a single carbon in galactose. FASEB J 10 : 461 470.
Gallant CV, Daniels C, Leung JM, Ghosh AS, Young KD, Kotra LP Burrows LL (2005) Common beta-lactamases inhibit bacterial biofilm formation. Mol Microbiol 58 : 1012 1024.
Gatz C, Altschmied J Hillen W (1986) Cloning and expression of the Acinetobacter calcoaceticus mutarotase gene in Escherichia coli. J Bacteriol 168 : 31 39.
Hecker M, Pane-Farre J Volker U (2007) SigB-dependent general stress response in Bacillus subtilis and related gram-positive bacteria. Annu Rev Microbiol 61 : 215 236.
Holden HM, Rayment I Thoden JB (2003) Structure and function of enzymes of the Leloir pathway for galactose metabolism. J Biol Chem 278 : 43885 43888.
Höltje JV (1995) From growth to autolysis: the murein hydrolases in Escherichia coli. Arch Microbiol 164 : 243 254.
Keck W Schwarz U (1979) Escherichia coli murein-dd-endopeptidase insensitive to beta-lactam antibiotics. J Bacteriol 139 : 770 774.
Korat B, Mottl H Keck W (1991) Penicillin-binding protein 4 of Escherichia coli: molecular cloning of the dacB gene, controlled overexpression, and alterations in murein composition. Mol Microbiol 5 : 675 684.
Krispin O Allmansberger R (1998) The Bacillus subtilis AraE protein displays a broad substrate specificity for several different sugars. J Bacteriol 180 : 3250 3252.
Lorca GL, Chung YJ, Barabote RD, Weyler W, Schilling CH Saier MH Jr. (2005) Catabolite repression and activation in Bacillus subtilis: dependency on CcpA, HPr, and HprK. J Bacteriol 187 : 7826 7839.
Nandy SK Venkatesh KV (2008) Effect of Carbon and Nitrogen on the cannibalistic behavior of Bacillus subtilis. Appl Biochem Biotech 151 : 424 432.
Nandy SK, Bapat PM Venkatesh KV (2007) Sporulating bacteria prefers predation to cannibalism in mixed cultures. FEBS Lett 581 : 151 156.
Pedersen LB, Murray T, Popham DL Setlow P (1998) Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis. J Bacteriol 180 : 4967 4973.
Ren D, Bedzyk LA, Setlow P, Thomas SM, Ye RW Wood TK (2004) Gene expression in Bacillus subtilis surface biofilms with and without sporulation and the importance of yveR for biofilm maintenance. Biotechnol Bioeng 86 : 344 364.
Sauvage E, Duez C, Herman R et al 2007) Crystal structure of the Bacillus subtilis penicillin-binding protein 4a, and its complex with a peptidoglycan mimetic peptide. J Mol Biol 371 : 528 539.
Sauvage E, Kerff F, Terrak M, Ayala JA Charlier P (2008) The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis. FEMS Microbiol Rev 32 : 234 258.
Stanley NR, Britton RA, Grossman AD Lazazzera BA (2003) Identification of catabolite repression as a physiological regulator of biofilm formation by Bacillus subtilis by use of DNA microarrays. J Bacteriol 185 : 1951 1957.
Thi Tam L, Eymann C, Antelmann H, Albrecht D Hecker M (2007) Global gene expression profiling of Bacillus subtilis in response to ammonium and tryptophan starvation as revealed by transcriptome and proteome analysis. J Mol Microb Biotech 12 : 121 130.
Thoden JB, Kim J, Raushel FM Holden HM (2002) Structural and kinetic studies of sugar binding to galactose mutarotase from Lactococcus lactis. J Biol Chem 277 : 45458 45465.
Tognoni A, Franchi E, Magistrelli C, Colombo E, Cosmina P Grandi G (1995) A putative new peptide synthase operon in Bacillus subtilis: partial characterization. Microbiology 141 : 645 648.
Yasukawa H, Kuroita T, Tamura K Yamaguchi K (2003) Identification of a penicillin-sensitive carboxypeptidase in the cellular slime mold Dictyostelium discoideum. Biol Pharm Bull 26 : 1018 1020.