Characterization of the proteins encoded by the Bacillus subtilis yoxA-dacC operon.

[en] Abstract In Bacillus subtilis, the yoxA and dacC genes were proposed to form an operon. The yoxA gene was overexpressed in Escherichia coli and its product fused to a polyhistidine tag was purified. An aldose-1-epimerase or mutarotase activity was measured with the YoxA protein that we propose to rename as GalM by analogy with its counterpart in E. coli. The peptide d-Glu-delta-m-A(2)pm-d-Ala-m-A(2)pm-d-Ala mimicking the B. subtilis and E. coli interpeptide bridge was synthesized and incubated with the purified dacC product, the PBP4a. A clear dd-endopeptidase activity was obtained with this penicillin-binding protein, or PBP. The possible role of this class of PBP, present in almost all bacteria, is discussed.

Research Center/Unit :

CIP - Centre d'Ingénierie des Protéines - ULiège
GIGA CRC (Cyclotron Research Center) In vivo Imaging-Aging & Memory - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Duez, Colette ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Zervosen, Astrid ; Université de Liège - ULiège > Centre de recherches du cyclotron

Teller, Nathalie; Université de Liège - ULiège > Centre de recherche du cyclotron

Melkonian, Rémy; Université de Liège - ULiège > Centre d'ingénierie des protéines

Banzubazé, Emmanuel

Bouillenne, Fabrice ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Luxen, André ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie organique de synthèse - Centre de recherches du cyclotron

Frère, Jean-Marie ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Language :

English

Title :

Characterization of the proteins encoded by the Bacillus subtilis yoxA-dacC operon.

Alternative titles :

[fr] Caractérisation des protéines codées par l'opéron yoxA-dacC de Bacillus subtilis

Publication date :

2009

Journal title :

FEMS Microbiology Letters

ISSN :

0378-1097

eISSN :

1574-6968

Publisher :

Blackwell Publishing, Oxford, United Kingdom

Volume :

300

Pages :

42-47

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

PAI, ARC et EUR-INTAFAR

Available on ORBi :

since 28 September 2009

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