Fungal diversity of “Tomme d'Orchies” cheese during the ripening process as revealed by a metagenomic study

Artisanal cheese; Fungal microbiota; Illumina; Cladosporium cladosporioides; Clavispora lusitaniae; Debaryomyces; Geotrichum candidum; Kazachstania unispora; Kluyveromyces; Yarrowia lipolytica; DNA sequence; France; Cheese; DNA, Ribosomal; Fungi; Genome, Fungal; High-Throughput Nucleotide Sequencing; Metagenomics; Sequence Analysis, DNA

Abstract :

[en] Tomme d'Orchies is an artisanal pressed and uncooked cheese produced and marketed in the north of France. This study aimed at showing the fungal microbiota evolution of this cheese using a metagenetic based Illumina technology targeting the ITS2 domain of 5.8S fungal rDNAs. To this end, samples were taken from the rind and the core of different cheeses, after 0, 1, 3, 7, 14 and 21 days of ripening. The data underpinned the prevalence of Yarrowia lipolytica and Galactomyces geotrichum for both microbiotas. Unusual species including Clavispora lusitaniae, Kazachstania unispora and Cladosporium cladosporioides were also detected, but their origins remain to be ascertained. The metagenomic revealed also the presence of Kluyveromyces and Debaryomyces species. © 2017 Elsevier B.V.

Disciplines :

Microbiology

Author, co-author :

Ceugniez, A.; Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 – ICV - Institut Charles Viollette, Lille, France

Taminiau, Bernard ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires

Coucheney, F.; Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 – ICV - Institut Charles Viollette, Lille, France

Jacques, Philippe ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies

Delcenserie, Véronique ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Gestion de la qualité dans la chaîne alimentaire

Daube, Georges ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires

Drider, D.; Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 – ICV - Institut Charles Viollette, Lille, France

Title :

Fungal diversity of “Tomme d'Orchies” cheese during the ripening process as revealed by a metagenomic study

Publication date :

2017

Journal title :

International Journal of Food Microbiology

ISSN :

0168-1605

eISSN :

1879-3460

Publisher :

Elsevier B.V.

Volume :

258

Pages :

89-93

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 13 August 2019

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