Article (Scientific journals)
Dynamic Remodeling of membrane composition drives cell cycle through primary cilia excision
Phua, Siew Cheng; Chiba, Shuhei; Suzuki, Masako et al.
2017In Cell, 168, p. 264-279
Peer Reviewed verified by ORBi
 

Files


Full Text
Inpp5e Cell 2017 2019.pdf
Publisher postprint (3.26 MB)
Correction: see https://doi.org/10.1016/j.cell.2010.06.015
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
primary cilia; Inpp5e; phosphoinositides
Abstract :
[en] The life cycle of a primary cilium begins in quiescence and ends prior to mitosis. In quiescent cells, the primary cilium insulates itself from contiguous dynamic membrane processes on the cell surface to function as a stable signaling apparatus. Here, we demonstrate that basal restriction of ciliary structure dynamics is established by the cilia-enriched phosphoinositide 5-phosphatase, Inpp5e. Growth induction displaces ciliary Inpp5e and accumulates phosphatidylinositol 4,5-bisphosphate in distal cilia. This change triggers otherwise-forbidden actin polymerization in primary cilia, which excises cilia tips in a process we call cilia decapitation. While cilia disassembly is traditionally thought to occur solely through resorption, we show that an acute loss of IFT-B through cilia decapitation precedes resorption. Finally, we propose that cilia decapitation induces mitogenic signaling and constitutes a molecular link between the cilia life cycle and cell-division cycle. This newly defined ciliary mechanism may find significance in cell proliferation control during normal development and cancer.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Phua, Siew Cheng
Chiba, Shuhei
Suzuki, Masako
Su, Emily
Roberson, Elle C.
Pusapati, Ganesh V.
Schurmans, Stéphane  ;  Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) > Biochimie métabolique vétérinaire
Setou, Mitsutoshi
Rohatgi, Rajat
Reiter, Jeremy F.
Ikegami, Koji
Inoue, Takanari
Language :
English
Title :
Dynamic Remodeling of membrane composition drives cell cycle through primary cilia excision
Publication date :
2017
Journal title :
Cell
ISSN :
0092-8674
eISSN :
1097-4172
Publisher :
Cell Press, United States - Massachusetts
Volume :
168
Pages :
264-279
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 14 June 2019

Statistics


Number of views
129 (19 by ULiège)
Number of downloads
103 (5 by ULiège)

Scopus citations®
 
225
Scopus citations®
without self-citations
213
OpenCitations
 
237

Bibliography


Similar publications



Contact ORBi