Article (Scientific journals)
Astin C production by the endophytic fungus Cyanodermella asteris in planktonic and immobilized culture conditions
Vassaux, Antoine; Tarayre, Cédric; Arguelles Arias, Anthony et al.
2019In Biotechnology Journal
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Keywords :
Astin C; Immobilized-cell culture; Cyanodermella asteris
Abstract :
[en] The fungal endophyte Cyanodermella asteris was recently isolated from the medicinal plant Aster tataricus. This fungus produces astin C, a cyclic pentapeptide with anticancer and anti-inflammatory properties. The production of this secondary metabolite was compared in immobilized and planktonic conditions. For immobilized cultures, a stainless steel packing immersed in the culture broth was used as a support. In these conditions, the fungus exclusively grew on the packing, which provides a considerable advantage for astin C recovery and purification. C. asteris metabolism was different according to the culture conditions in terms of substrate consumption rate, cell-growth, and astin C production. Immobilized-cell cultures yielded a 30% increase of astin C production associate to a 39% increase in biomass. The inoculum type as spores rather than hyphae, and a pre-inoculation washing procedure with sodium hydroxide, turned out to be beneficial both for astin C production and fungus development onto the support. Finally, influence of culture parameters such as pH and medium composition, on astin C production was evaluated. With optimized culture conditions, astin C yield was further improved reaching a five times higher final specific yield compared to the value reported with astin C extraction from Aster tataricus (0.89 and 0.16 mg/g respectively).
Disciplines :
Biotechnology
Author, co-author :
Vassaux, Antoine ;  Université de Liège - ULiège > Doct. sc. agro. & ingé. biol. (Paysage)
Tarayre, Cédric
Arguelles Arias, Anthony ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Compère, Philippe ;  Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Morphologie fonctionnelle et évolutive
Delvigne, Frank  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Fickers, Patrick ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Jahn, Linda
Lang, Alexander
Leclère, Valérie
Ludwig-Müller, Jutta
Ongena, Marc ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Schafhauser, Thomas
Telek, Samuel  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Théatre, Ariane ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
van Berkel, Willem
Vandenbol, Micheline ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
van Pée, Karl-Heinz
Willems, Luc  ;  Université de Liège - ULiège > Cancer-Cellular and Molecular Epigenetics
Wohlleben, Wolfgang
Jacques, Philippe ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
More authors (10 more) Less
Language :
English
Title :
Astin C production by the endophytic fungus Cyanodermella asteris in planktonic and immobilized culture conditions
Publication date :
04 June 2019
Journal title :
Biotechnology Journal
ISSN :
1860-6768
eISSN :
1860-7314
Publisher :
Wiley - VCH Verlag GmbH & Co. KGaA, Germany
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 12 June 2019

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