High-level biosynthesis of the anteiso-C(17) isoform of the antibiotic mycosubtilin in Bacillus subtilis and characterization of its candidacidal activity.

Fickers, Patrick; Guez, Jean-Sebastien; Damblon, Christian; Leclere, Valerie; Bechet, Max; Jacques, Philippe; Joris, Bernard

doi:10.1128/AEM.00548-09

Article (Scientific journals)

High-level biosynthesis of the anteiso-C(17) isoform of the antibiotic mycosubtilin in Bacillus subtilis and characterization of its candidacidal activity.

Fickers, Patrick; Guez, Jean-Sebastien; Damblon, Christian et al.

2009 • In Applied and Environmental Microbiology, 75 (13), p. 4636-40

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/23494

DOI
10.1128/AEM.00548-09

PubMed
19429561

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Keywords :

Antibiotics, Antifungal/biosynthesis; Bacillus subtilis/genetics/metabolism; Biosynthetic Pathways/genetics; Candida/drug effects; Genes, Bacterial; Genetic Engineering; Lipoproteins/biosynthesis; Microbial Sensitivity Tests; Protein Isoforms/metabolism

Abstract :

[en] High-level production (880 mg liter(-1)) and isolation of the anteiso-C(17) isoform of the lipopeptide mycosubtilin produced by a genetically engineered Bacillus subtilis strain are reported. Antifungal activity of this isoform, as determined via culture and fluorometric and cell leakage assays, suggests its potential therapeutic use as an antifungal agent, in particular against Candida spp.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Fickers, Patrick ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Guez, Jean-Sebastien

Damblon, Christian ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie biologique structurale

Leclere, Valerie

Bechet, Max

Jacques, Philippe ; Université de Liège - ULiège > Chimie et bio-industries > Bio-industries

Joris, Bernard ; Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes - Centre d'ingénierie des protéines

Language :

English

Title :

High-level biosynthesis of the anteiso-C(17) isoform of the antibiotic mycosubtilin in Bacillus subtilis and characterization of its candidacidal activity.

Publication date :

2009

Journal title :

Applied and Environmental Microbiology

ISSN :

0099-2240

eISSN :

1098-5336

Publisher :

American Society for Microbiology (ASM), Washington, United States - District of Columbia

Volume :

Issue :

Pages :

4636-40

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 23 September 2009

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Bibliography

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