Article (Scientific journals)
Engineering Molecular Immunity Against Plant Viruses.
Syed-Shan-e-Ali, Zaïdi; Tashkandi, Manal; Mahfouz, Magdy M.
2017In Progress in Molecular Biology and Translational Science, 149, p. 167-186
Peer reviewed
 

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Keywords :
CRISPR-Cas Systems/genetics; Disease Resistance/immunology; Genetic Engineering/methods; Plant Diseases/genetics/immunology/virology; Plant Immunity/genetics; Plant Viruses/physiology; CRISPR/Cas9; Geminiviruses; Genome engineering; Site-specific nuclease; Tobacco rattle virus
Abstract :
[en] Genomic engineering has been used to precisely alter eukaryotic genomes at the single-base level for targeted gene editing, replacement, fusion, and mutagenesis, and plant viruses such as Tobacco rattle virus have been developed into efficient vectors for delivering genome-engineering reagents. In addition to altering the host genome, these methods can target pathogens to engineer molecular immunity. Indeed, recent studies have shown that clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) systems that target the genomes of DNA viruses can interfere with viral activity and limit viral symptoms in planta, demonstrating the utility of this system for engineering molecular immunity in plants. CRISPR/Cas9 can efficiently target single and multiple viral infections and confer plant immunity. Here, we discuss the use of site-specific nucleases to engineer molecular immunity against DNA and RNA viruses in plants. We also explore how to address the potential challenges encountered when producing plants with engineered resistance to single and mixed viral infections.
Disciplines :
Genetics & genetic processes
Biotechnology
Biochemistry, biophysics & molecular biology
Agriculture & agronomy
Author, co-author :
Syed-Shan-e-Ali, Zaïdi ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Ingénierie des productions végétales et valorisation
Tashkandi, Manal
Mahfouz, Magdy M.
Language :
English
Title :
Engineering Molecular Immunity Against Plant Viruses.
Publication date :
2017
Journal title :
Progress in Molecular Biology and Translational Science
ISSN :
1877-1173
eISSN :
1878-0814
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
149
Pages :
167-186
Peer reviewed :
Peer reviewed
Available on ORBi :
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