Rapid SPE-HPLC determination of the 16European priority polycyclic aromatic hydrocarbons in olive oils

European legislation; HPLC; Olive oils; Polycyclic aromatic hydrocarbons (PAHs); SPE; Olive oil; Polycyclic aromatic hydrocarbons(PAHs); High performance liquid chromatography; Laws and legislation; Vegetable oils; Polycyclic aromatic hydrocarbons; Europe; Chromatography, High Pressure Liquid; Plant Oils; Polycyclic Compounds; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Spectrophotometry, Ultraviolet

Abstract :

[en] Polycyclic aromatic hydrocarbons (PAHs) are a large class of organic compounds. It has been established that the main source of exposure to these compounds for human beings is through food, particularly fats and oils, due to the lipophilic nature of these polycyclic compounds. The aim of this work was to optimise and validate a method involving SPE and HPLC for rapid determination of the 16 European Union (EU) priority PAHs (required by the Recommendation 2005/108/EC) in vegetable oils. Two spectrofluorometric detectors and a UV-Visible detector in series were used to identify and quantify the target compounds. Linearity, recoveries, LOD, and LOQ were found to be in agreement with the performance criteria for ben-zo[a]pyrene (BaP) analysis as required by the Commission Directive 2005/10/EC, and satisfactory for all the compounds of interest, except for cyclopenta [c,d] pyrene, which presented a very low signal in the UV. Optimised chromatographic conditions for the separation of 25 PAHs, comprising both EPA and EU priority PAHs plus benzo[e]pyrene and benzo[b]chrysene, have been also proposed. © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Disciplines :

Chemistry
Food science

Author, co-author :

Purcaro, Giorgia ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes

Moret, S.; Department of Food Science, University of Udine, via Sondrio 2, 33100 Udine, Italy

Conte, L. S.; Department of Food Science, University of Udine, via Sondrio 2, 33100 Udine, Italy

Language :

English

Title :

Rapid SPE-HPLC determination of the 16European priority polycyclic aromatic hydrocarbons in olive oils

Publication date :

2008

Journal title :

Journal of Separation Science

ISSN :

1615-9306

eISSN :

1615-9314

Publisher :

Wiley, Weinheim, Germany

Volume :

Issue :

Pages :

3936-3944

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 10 January 2019

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