Article (Scientific journals)
Preservation of Cortical Microcirculation after Kidney Ischemia-Reperfusion: Value of an Iron Chelator
Defraigne, Jean-Olivier; Pincemail, Joël; Detry, Olivier et al.
1994In Annals of Vascular Surgery, 8 (5), p. 457-67
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Abstract :
[en] Treatment of suprarenal aneurysms and renal artery reconstructions are both responsible for normothermic ischemia of the kidney (during clamping) followed by reperfusion (declamping). During reflow through an organ undergoing ischemia the production of free radicals can be associated with cell injury and a no-reflow phenomenon characterized by perfusion defects after a period of transient hyperemia. The objectives of this study were to demonstrate the existence of this phenomenon in the kidney undergoing ischemia followed by reperfusion and to test the potential protection afforded by an iron chelator (desferrioxamine) since free radical reactions are catalyzed by iron. Adult New Zealand white rabbits were divided into the following three groups: group A, 15 minutes of ischemia plus 10 minutes of reperfusion; group B, 60 minutes of ischemia plus 10 minutes of reperfusion; and group C, 60 minutes of ischemia plus 10 minutes of reperfusion combined with infusion of desferrioxamine (50 mg/kg). Cortical microcirculation in the kidney was measured by laser Doppler flowmeter before ischemia and 1, 5, and 10 minutes after reperfusion. Vitamin E content was determined in the cortex of the left kidney after 10 minutes of reperfusion and compared with that of the right (control) kidney. After 1 minute of reperfusion the cortical microcirculatory flow was significantly increased in all three groups (reactive hyperemia). In groups A and C blood flow returned to preclamping values after 10 minutes of reperfusion; however, blood flow in group B remained significantly reduced (29.2% +/- 10.5%) after 5 minutes of reperfusion with a further reduction to 48.5% +/- 5.7% after 10 minutes. These findings were correlated with the dosage of vitamin E since the vitamin E content was greatly reduced by 46.7% +/- 7.8% in group B but did not change significantly in groups A and C. This study shows that 60 minutes of normothermic ischemia is followed by a significant reduction in cortical microcirculatory flow (no-reflow phenomenon). Infusion of an iron chelator (desferrioxamine), however, which decreases the intensity of lipid peroxidation induced by the free radicals, preserves the microcirculatory flow.
Disciplines :
Cardiovascular & respiratory systems
Immunology & infectious disease
Surgery
Author, co-author :
Defraigne, Jean-Olivier ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie générale
Pincemail, Joël ;  Centre Hospitalier Universitaire de Liège - CHU > Chirurgie cardio-vasculaire
Detry, Olivier  ;  Centre Hospitalier Universitaire de Liège - CHU > Chirurgie abdominale- endocrinienne et de transplantation
Franssen, Christine ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires
Meurisse, Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Chirurgicale abdominale
Limet, Raymond ;  Université de Liège - ULiège > Département des sciences cliniques > Chirurgie cardio-vasculaire et thoracique
Language :
English
Title :
Preservation of Cortical Microcirculation after Kidney Ischemia-Reperfusion: Value of an Iron Chelator
Publication date :
September 1994
Journal title :
Annals of Vascular Surgery
ISSN :
0890-5096
eISSN :
1615-5947
Publisher :
Springer, Germany
Volume :
8
Issue :
5
Pages :
457-67
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 21 September 2009

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