Reference : Growth hormone (GH) deficient mice with GHRH ablation are severely deficient in vacci...
Scientific journals : Article
Human health sciences : Neurology
Human health sciences : Endocrinology, metabolism & nutrition
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/228843
Growth hormone (GH) deficient mice with GHRH ablation are severely deficient in vaccine and immune responses against Streptococcus pneumoniae
English
Farhat, Khalil mailto [Université de Liège - ULiège > GIGA-I3 > Immunoendocrinologie > >]
Bodart, Gwennaëlle mailto [Université de Liège - ULiège > > GIGA-R : Hématologie >]
RENARD, Jeanne de Chantal [Centre Hospitalier Universitaire de Liège - CHU > Département ULG > GIGA >]
Desmet, Christophe mailto [Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire >]
Moutschen, Michel mailto [Université de Liège - ULiège > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
Beguin, Yves mailto [Université de Liège - ULiège > > GIGA-R : Hématologie >]
Baron, Frédéric mailto [Université de Liège - ULiège > > GIGA-R : Hématologie >]
Melin, Pierrette mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Bactériologie, mycologie, parasitologie, virologie >]
Quatresooz, Pascale mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie >]
Parent, Anne-Simone mailto [Université de Liège - ULiège > Département des sciences cliniques > Pédiatrie >]
Desmecht, Daniel mailto [Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Pathologie spéciale et autopsies >]
Sirard, Jean-Claude mailto [Institut Pasteur Lille > > > >]
Salvatori, Roberto mailto [Johns Hopkins > > > >]
Martens, Henri mailto [Université de Liège - ULiège > > Centre d'immunologie >]
Geenen, Vincent mailto [Université de Liège - ULiège > > Centre d'immunologie >]
2-Oct-2018
Frontiers in Immunology
Frontiers Media S.A.
9
Article 2175
No
International
1664-3224
Switzerland
[en] Somatotrope axis ; GHRH ; GH ; Sreptococcus pneumoniae ; Thymo-independent antigen ; Spleen
[en] The precise impact of the somatotrope axis upon the immune system is still highly debated. We have previously shown that mice with generalized ablation of growth hormone (GH) releasing hormone (GHRH) gene (Ghrh−/−) have normal thymus and T-cell development, but present a marked spleen atrophy and B-cell lymphopenia. Therefore, in this paper we have investigated vaccinal and anti-infectious responses of Ghrh−/− mice against S. pneumoniae, a pathogen carrying T-independent antigens. Ghrh−/− mice were unable to trigger production of specific IgM after vaccination with either native pneumococcal polysaccharides (PPS, PPV23) or protein-PPS conjugate (PCV13). GH supplementation of Ghrh−/− mice restored IgM response to PPV23 vaccine but not to PCV13 suggesting that GH could exert a specific impact on the spleen marginal zone that is strongly implicated in T-independent response against pneumococcal polysaccharides. As expected, after administration of low dose of S. pneumoniae, wild type (WT) completely cleared bacteria after 24 h. In marked contrast, Ghrh−/− mice exhibited a dramatic susceptibility to S. pneumoniae infection with a time-dependent increase in lung bacterial load and a lethal bacteraemia already after 24 h. Lungs of infected Ghrh−/− mice were massively infiltrated by inflammatory macrophages and neutrophils, while lung B cells were markedly decreased. The inflammatory transcripts signature was significantly elevated in Ghrh−/− mice. In this animal model, the somatotrope GHRH/GH/IGF1 axis plays a vital and unsuspected role in vaccine and immunological defense against S. pneumoniae.
Giga-Infection, Immunity and Inflammation
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds Léon Fredericq ; Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE ; Fédération Wallonie-Bruxelles (ARC Somasthym) ; Association of Specialization and Scientific Guidance (Lebanon)
SOMASTHYM
Researchers ; Professionals
http://hdl.handle.net/2268/228843
10.3389/fimmu.2018.02175
https://www.frontiersin.org/articles/10.3389/fimmu.2018.02175/full?&utm_source=Email_to_authors_&utm_medium=Email&utm_content=T1_11.5e1_author&utm_campaign=Email_publication&field=&journalName=Frontiers_in_Immunology&id=411122

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