Bacteriocin-like substances; Listeria monocytogenes; Enterococcus spp.; Peruvian traditional cheese
Résumé :
[en] The aim of this work was to isolate and to characterize strains of lactic acid bacteria (LAB) with bacteriocin-like inhibitory activity from 27 traditional cheeses artisanal-produced obtained from different Peruvian regions. Twenty Gram+ and catalasenegative strains among 2,277 isolates exhibited bacteriocin-like inhibitory activity against Listeria monocytogenes CWBIB2232 as target strain. No change in inhibitory activity was observed after organic acid neutralization and treatment with catalase of the cell-free supernatant (CFS). The proteinic nature of the antimicrobial activity was confirmed for the twenty LAB strains by proteolytic digestion of the CFS. Two strains, CWBI-B1431 and CWBI-B1430, with the best antimicrobial activity were selected for further researches. These strains were taxonomically identified by phenotypic and genotypic analyses as Enterococcus mundtii (CWBI-B1431) and Enterococcus faecium (CWBI-B1430). The two strains were sensitive to vancomycin (MIC < 2 μg.ml-1) and showed absence of haemolysis.
Ananou, S., Control of Listeria monocytogenes in model sausages by enterocin AS-48 (2005) Int. J. Food Microbiol., 103, pp. 179-190
Aymerich, T., Effect of sausage ingredients and additives on the production of enterocins A and B by Enterococcus faecium CTC492. Optimization of in vitro production and anti-listerial effect in dry fermented sausages (2000) J. Appl. Microbiol., 88, pp. 686-694
Benkerroum, N., Isolation of a bacteriocinproducing Lactococcus lactis subsp. lactis and appli-cation to control Listeria monocytogenes in Moroccan jben (2000) J. Appl. Microbiol., 89, pp. 960-968
Bennik, M., A novel bacteriocin with a YGNGV motif from vegetable-associated Enterococcus mundtii: Full characterization and interaction with target organisms (1998) Biochim. Biophys. Acta, 1373, pp. 47-58
Campos, C., Preliminary characterization of bacteriocins from Lactococcus lactis, Enterococcus faecium and Enterococcus mundtii strains isolated from turbot (Psetta maxima) (2006) Food Res. Int., 39, pp. 356-364
Casaus, P., Enterocin B, a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin A (1997) Microbiology, 143, pp. 2287-2294
Cheng, H., Hoover, D., Bacteriocins and their food applications (2003) Comp. Rev. Food Sci. Food Saf., 2, pp. 82-100
Cintas, L., Biochemical and genetic characterization of Enterocin P, a novel sec-dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum (1997) Appl. Environ. Microbiol., 63 (11), pp. 4321-4330
Cintas, L., Casaus, P., Fernández, M., Hernández, P., Comparative antimicrobial activity of enterocin L50, pediocin PA-1, nisin A and lactocin S against spoilage and foodborne pathogenic bacteria (1998) Food Microbiol., 15, pp. 289-298
Criado, R., Immunochemical characterization of temperature-regulated production of enterocin L50 (EntL50A and EntL50B), enterocin P, and enterocin Q by Enterococcus faecium L50 (2006) Appl. Environ. Microbiol., 72 (12), pp. 7634-7643
de Kwaadsteniet, M., Todorov, S., Knoetze, H., Dicks, L., Characterization of a 3944 Da bacteriocin, produced by Enterococcus mundtii ST15, with activity against Gram+ and Gram- bacteria (2005) Int. J. Food Microbiol., 105, pp. 433-444
Diop, M., Bacteriocin producers from traditional food products (2007) Biotechnol. Agron. Soc. Environ., 11 (4), pp. 275-281
Foulquié, M., Isolation and biochemical characterization of enterocins produced by enterococci from different sources (2003) J. Appl. Microbiol., 94, pp. 214-229
Franz, C., Diversity of enterococcal bacteriocins and their grouping in a new classification scheme (2007) FEMS Microbiol. Rev., 31 (3), pp. 293-310
Franzetti, L., Pompei, M., Scarpellini, M., Galli, A., Phenotypic and genotypic characterisation of Enterococcus spp. of different origins (2004) Curr. Microbiol., 49, pp. 255-260
Garriga, M., Bactericidal synergism through bacteriocins and high pressure in a meat model system during storage (2002) Food Microbiol., 19, pp. 509-518
Ghrairi, T., Frere, J., Berjeaud, J., Manai, M., Purification and characterisation of bacteriocins produced by Enterococcus faecium from Tunisian rigouta cheese (2008) Food Control, 19, pp. 162-169
Giraffa, G., Functionality of enterococci in dairy products (2003) Int. J. Food Microbiol., 88, pp. 215-222
Herranz, C., Enterococcus faecium P21: A strain occurring naturally in dry-fermented sausages producing the class II bacteriocins enterocin A and enterocin B (2001) Food Microbiol., 18, pp. 115-131
Higashide, T., Endophthalmitis caused by Enterococcus mundtii (2005) J. Clinical Microbiol., 43 (3), pp. 1475-1476
Kaufhold, A., Ferrieri, P., Isolation of Enterococcus mundtii from normally sterile body sites in two patients (1991) J. Clinical Microbiol., 29 (5), pp. 1075-1077
Klaenhammer, T., Genomic features of lactic acid bacteria effecting bioprocessing and health (2005) FEMS Microbiol. Rev., 29, pp. 393-409
Manero, A., Blanch, A., Identification of Enterococcus spp. with a biochemical key (1999) Appl. Environ. Microbiol., 65 (10), pp. 4425-4430
Marino, M., Maifreni, M., Rondinini, G., Microbiological characterization of artisanal Montasio cheese: Analysis of its indigenous lactic acid bacteria (2003) FEMS Microbiol. Lett., 229, pp. 133-140
Morea, M., Baruzzi, F., Cocconcelli, P., Molecular and physiological characterization of dominant bacterial populations in traditional Mozzarella cheese processing (1999) J. Appl. Microbiol., 87, pp. 574-582
Muñoz-Rojas, J., Bacteriocinas: Una estrategia de competencia microbiana propuesta como alternativa de antibióticos dirigidos para el futuro humano (2008) Microbio en línea, , www.microbiologia.org-mx/microbiosenlinea, In: Martínez E. & Martínez J., eds, (28/07/08)
O'sullivan, L., Ross, R., Hill, C., Potential of bacteriocin-producing lactic acid bacteria for improvements in food safety and quality (2002) Biochimie, 84, pp. 593-604
Ocaña, V., Pesce, A., Nader-Macías, N., Characterization of a bacteriocin-like substance produced by a vaginal Lactobacillus salivarius strain (1999) Appl. Environ. Microbiol., 65, pp. 5631-5635
Pedron, E., Niederauer, R., Salete, R., Paiva, M., Bacteriocin-like substance of Aeromonas hydrophila (1997) Mem. Inst. Oswaldo Cruz, 92 (1), pp. 115-116
Sarantinopoulos, P., Bacteriocin production by Enterococcus faecium FAIR-E 198 in view of its application as adjunct starter in Greek Feta cheese making (2002) Int. J. Food Microbiol., 72, pp. 125-136
Schillinger, U., Lucke, F., Antibacterial activity of Lactobacillus sake isolated from meat (1989) Appl. Environ. Microbiol., 55 (8), pp. 1901-1906
van Belkum, M., Stiles, M., Nonlantibiotic antibacterial peptides from lactic acid bacteria (2000) Nat. Prod. Rep., 17, pp. 323-365
