Reference : Description of two Enterococcus strains isolated from traditional Peruvian artisanal-...
Scientific journals : Article
Life sciences : Microbiology
Description of two Enterococcus strains isolated from traditional Peruvian artisanal-produced cheeses with a bacteriocin-like inhibitory activity
Aguilar Galvez, Ana Consuelo [Université de Liège - ULiège > > > Doct. sc. agro. & ingé. biol.]
Dubois Dauphin, Robin [Université de Liège - ULiège > > Gembloux Agro-Bio Tech >]
Ghalfi, Hakim [> >]
Campos Gutiérrez, David Carlos [Universidad Nacional Agraria La Molina > > Instituto de Biotecnología > >]
Thonart, Philippe mailto [Université de Liège - ULiège > > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles >]
Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE]
Presses Agronomiques de Gembloux
Yes (verified by ORBi)
[en] Bacteriocin-like substances ; Listeria monocytogenes ; Enterococcus spp. ; Peruvian traditional cheese
[en] The aim of this work was to isolate and to characterize strains of lactic acid bacteria (LAB) with bacteriocin-like inhibitory activity from 27 traditional cheeses artisanal-produced obtained from different Peruvian regions. Twenty Gram+ and catalasenegative strains among 2,277 isolates exhibited bacteriocin-like inhibitory activity against Listeria monocytogenes CWBIB2232 as target strain. No change in inhibitory activity was observed after organic acid neutralization and treatment with catalase of the cell-free supernatant (CFS). The proteinic nature of the antimicrobial activity was confirmed for the twenty LAB strains by proteolytic digestion of the CFS. Two strains, CWBI-B1431 and CWBI-B1430, with the best antimicrobial activity were selected for further researches. These strains were taxonomically identified by phenotypic and genotypic analyses as Enterococcus mundtii (CWBI-B1431) and Enterococcus faecium (CWBI-B1430). The two strains were sensitive to vancomycin (MIC < 2 μ and showed absence of haemolysis.

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