[en] A straightforward analytical method for the determination of estrone (E1) and 17β-estradiol (E2) at ultra-low levels in serum samples by LC-MS/MS has been developed and validated. This method entails an extraction and derivatization with dansyl chloride followed by separation with a C18 column. A comparison of the developed LC-MS/MS method against our routine immunoassay shows a good correlation for E2 while an important negative bias is observed for E1 by RIA. The later comparison for E1 shows the need to switch from the current routine automated immunoassays to highly-sensitive LC-MS/MS quantifications in order to provide accurate and reliable clinical results, especially at very low levels.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Pitarch-Motellón, Jorge; University Jaume I > Analytical Chemistry
Cavalier, Etienne ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
LE GOFF, Caroline ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire techniques séparatives et stress oxydant
PEETERS, Stéphanie ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire techniques séparatives et stress oxydant
Fabregat Cabello, Neus ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Language :
English
Title :
Development and Validation of the Simultaneous Measurement of Estrone and 17β-Estradiol in Serum by LC-MS/MS for Clinical Laboratory Applications
Publication date :
12 September 2018
Event name :
MSACL EU 2018
Event organizer :
The Association for Mass Spectrometry: Applications to the Clinical Lab (MSACL)
