[en] Background and objectives: The kidney stone’s structure might provide clinical information in addition to the stone composition. The Raman chemical imaging is a technology used for the production of two-dimension maps of the constituents' distribution in samples. We aimed at determining the use of Raman chemical imaging in urinary stone analysis.
Material and methods: Fourteen calculi were analyzed by Raman chemical imaging using a confocal Raman microspectrophotometer. They were selected according to their heterogeneous composition and morphology. Raman chemical imaging was performed on the whole section of stones. Once acquired, the data were baseline corrected and analyzed by MCR-ALS. Results were then compared to the spectra obtained by Fourier Transform Infrared spectroscopy.
Results: Raman chemical imaging succeeded in identifying almost all the chemical components of each sample, including monohydrate and dihydrate calcium oxalate, anhydrous and dihydrate uric acid, apatite, struvite, brushite, and rare chemicals like whitlockite, ammonium urate and drugs. However, proteins couldn't be detected because of the huge autofluorescence background and the small concentration of these poor Raman scatterers. Carbapatite and calcium oxalate were correctly detected even when they represented less than 5 percent of the whole stones. Moreover, Raman chemical imaging provided the distribution of components within the stones: nuclei were accurately identified, as well as thin layers of other components. Conversion of dihydrate to monohydrate calcium oxalate was correctly observed in the centre of one sample. The calcium oxalate monohydrate had different Raman spectra according to its localization.
Conclusion: Raman chemical imaging showed a good accuracy in comparison with infrared spectroscopy in identifying components of kidney stones. This analysis was also useful in determining the organization of components within stones, which help locating constituents in low quantity, such as nuclei. However, this analysis is time-consuming, making it more suitable for research studies rather than routine analysis.
Disciplines :
Laboratory medicine & medical technology Urology & nephrology
Author, co-author :
Castiglione, Vincent ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Sacre, Pierre-Yves ; Université de Liège - ULiège > Département de pharmacie > Chimie analytique
CAVALIER, Etienne ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Service de chimie clinique
Hubert, Philippe ; Université de Liège - ULiège > Département de pharmacie > Chimie analytique
GADISSEUR, Romy ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire biochimie automatisée et analyses délocalisées
Ziemons, Eric ; Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Language :
English
Title :
Raman Chemical Imaging, a new tool in kidney stone structure analysis: Case-study and comparison to Fourier Transform Infrared spectroscopy
Alternative titles :
[fr] La microscopie Raman, un nouvel outil pour l'analyse structurelle des calculs urinaires: étude de cas et comparaison à la scpectroscopie infrarouge à transformée de Fourrier
Publication date :
03 August 2018
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science, United States - California
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