Development of real-time PCR tests for the detection of Tenebrio molitor in food and feed

Insect; Tenebrio molitor; Animals; Error detection; Genes; Proteins; Limit of detection; Performance criterion; Processed animal proteins; Qualitative method; Real-time PCR; Real-time PCR method; Polymerase chain reaction; DNA; DNA 18S; RNA 18S; RNA 28S; Article; Bombyx mori; Gammarus; Zophobas morio; Tenebrio; Animal Feed; Food Analysis; Real-Time Polymerase Chain Reaction

Abstract :

[en] Insects are rich in proteins and could be an alternative source of proteins to feed animals and humans. Numerous companies have started the production of insects for feed purposes. In Europe, these processed animal proteins are not yet authorised by legislation as many questions still need to be answered concerning this ‘novel food’. Authorisations will be possible when methods of authentication of the products are available. In this study we propose real-time PCR methods for the specific detection of the mealworm (Tenebriomolitor), one of the most widely used insects for food and feed production. Two PCR assays are proposed: the first based on the wingless gene and the second based on the cadherin gene. The PCR tests amplify fragments of 87 bp. These qualitative methods were tested according to several performance criteria. The specificity was tested on 34 insect species’ DNA, but also on non-insect species including crustacean, mammals, birds and plants. The limit of detection was determined and was below 20 copies for the two PCR tests. The applicability of the tests was demonstrated by the analysis of real-life processed samples containing T. molitor. © 2017 Informa UK Limited, trading as Taylor & Francis Group.

Disciplines :

Food science

Author, co-author :

Debode, F.; Unit Traceability and Authentication, Walloon Agricultural Research Center (CRA-W), Gembloux, Belgium

Marien, A.; Unit Traceability and Authentication, Walloon Agricultural Research Center (CRA-W), Gembloux, Belgium, European Union Reference Laboratory for Animal Proteins in feedingstuffs (EURL-AP), Gembloux, Belgium

Gerard, Amaury ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes

Francis, Frédéric ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Gestion durable des bio-agresseurs

Fumière, O.; Unit Traceability and Authentication, Walloon Agricultural Research Center (CRA-W), Gembloux, Belgium, European Union Reference Laboratory for Animal Proteins in feedingstuffs (EURL-AP), Gembloux, Belgium

Berben, G.; Unit Traceability and Authentication, Walloon Agricultural Research Center (CRA-W), Gembloux, Belgium, European Union Reference Laboratory for Animal Proteins in feedingstuffs (EURL-AP), Gembloux, Belgium

Language :

English

Title :

Development of real-time PCR tests for the detection of Tenebrio molitor in food and feed

Publication date :

2017

Journal title :

Food Additives and Contaminants. Part A. Chemistry, Analysis, Control, Exposure and Risk Assessment

ISSN :

1944-0049

eISSN :

1944-0057

Publisher :

Taylor & Francis, United States

Volume :

Issue :

Pages :

1421-1426

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 19 August 2018

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