Reference : Development of real-time PCR tests for the detection of Tenebrio molitor in food and feed
Scientific journals : Article
Life sciences : Food science
http://hdl.handle.net/2268/227173
Development of real-time PCR tests for the detection of Tenebrio molitor in food and feed
English
Debode, F. mailto [Unit Traceability and Authentication, Walloon Agricultural Research Center (CRA-W), Gembloux, Belgium]
Marien, A. [Unit Traceability and Authentication, Walloon Agricultural Research Center (CRA-W), Gembloux, Belgium, European Union Reference Laboratory for Animal Proteins in feedingstuffs (EURL-AP), Gembloux, Belgium]
Gerard, Amaury mailto [Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes >]
Francis, Frédéric mailto [Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Gestion durable des bio-agresseurs >]
Fumière, O. mailto [Unit Traceability and Authentication, Walloon Agricultural Research Center (CRA-W), Gembloux, Belgium, European Union Reference Laboratory for Animal Proteins in feedingstuffs (EURL-AP), Gembloux, Belgium]
Berben, G. [Unit Traceability and Authentication, Walloon Agricultural Research Center (CRA-W), Gembloux, Belgium, European Union Reference Laboratory for Animal Proteins in feedingstuffs (EURL-AP), Gembloux, Belgium]
2017
Food Additives and Contaminants. Part A. Chemistry, Analysis, Control, Exposure and Risk Assessment
Taylor & Francis
34
8
1421-1426
Yes (verified by ORBi)
International
1944-0049
1944-0057
United States
[en] Insect ; Tenebrio molitor ; Animals ; Error detection ; Genes ; Proteins ; Limit of detection ; Performance criterion ; Processed animal proteins ; Qualitative method ; Real-time PCR ; Real-time PCR method ; Polymerase chain reaction ; DNA ; DNA 18S ; RNA 18S ; RNA 28S ; Article ; Bombyx mori ; Gammarus ; Zophobas morio ; Tenebrio ; Animal Feed ; Animals ; Food Analysis ; Real-Time Polymerase Chain Reaction ; Tenebrio
[en] Insects are rich in proteins and could be an alternative source of proteins to feed animals and humans. Numerous companies have started the production of insects for feed purposes. In Europe, these processed animal proteins are not yet authorised by legislation as many questions still need to be answered concerning this ‘novel food’. Authorisations will be possible when methods of authentication of the products are available. In this study we propose real-time PCR methods for the specific detection of the mealworm (Tenebriomolitor), one of the most widely used insects for food and feed production. Two PCR assays are proposed: the first based on the wingless gene and the second based on the cadherin gene. The PCR tests amplify fragments of 87 bp. These qualitative methods were tested according to several performance criteria. The specificity was tested on 34 insect species’ DNA, but also on non-insect species including crustacean, mammals, birds and plants. The limit of detection was determined and was below 20 copies for the two PCR tests. The applicability of the tests was demonstrated by the analysis of real-life processed samples containing T. molitor. © 2017 Informa UK Limited, trading as Taylor & Francis Group.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/227173
10.1080/19440049.2017.1320811

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
Tenebrio 2017.pdfAuthor postprint599.08 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.