Interbreed variation in serum serotonin (5-hydroxytryptamine) concentration in healthy dogs.

[en] INTRODUCTION: Serotonin (5-hydroxytryptamine [5-HT]) has several biological functions. In different species, excessive 5-HT has been linked to valvular lesions, similar to those seen in dogs with myxomatous mitral valve disease. Previous studies suggest higher 5-HT in healthy Cavalier King Charles Spaniels (CKCSs), a breed highly affected by myxomatous mitral valve disease, compared to other breeds. OBJECTIVE: To investigate potential interbreed variation in serum 5-HT in healthy dogs. ANIMALS: 483 healthy dogs of nine breeds aged 1-7 years. METHODS: Dogs were examined at five European centers. Absence of cardiovascular, organ-related, or systemic diseases was ensured by thorough clinical investigations including echocardiography. Serum was frozen and later analyzed by enzyme-linked immunosorbent assay (ELISA). RESULTS: Median 5-HT concentration was 252.5 (interquartile range = 145.5-390.6) ng/mL. Overall breed difference was found (p<0.0001), and 42% of pairwise breed comparisons were significant. Univariate regression analysis showed association between serum 5-HT concentration and breed, center of examination, storage time, and sex, with higher 5-HT in females. In multiple regression analysis, the final model had an adjusted R2 of 0.27 with breed (p<0.0001), center (p<0.0001), and storage time (p=0.014) remaining significant. Within centers, overall breed differences were found at 3/5 centers (p≤0.028), and pairwise comparisons within those centers showed breed differences in 42% of comparisons. Among the included breeds, Newfoundlands, Belgian Shepherds and CKCSs had highest 5-HT concentrations. CONCLUSIONS: Interbreed variation in serum 5-HT concentration was found in healthy dogs aged 1-7 years. These differences should be taken into account when designing clinical studies.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Höglund, Katja

Häggström, Jens

Hanas, S

Merveille, Anne-Christine ; Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Pathologie médicale des petits animaux

Gouni, Vassiliki

Wiberg, Maria

Lundgren Willesen, Jacob

Mc Entee, Kathleen ; Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Pathologie médicale des petits animaux

Mejer Sorensen, Louise

Tiret, Laurent

More authors (6 more)

Language :

English

Title :

Interbreed variation in serum serotonin (5-hydroxytryptamine) concentration in healthy dogs.

Publication date :

June 2018

Journal title :

Journal of Veterinary Cardiology

ISSN :

1760-2734

eISSN :

1875-0834

Publisher :

Elsevier, Netherlands

Peer reviewed :

Peer reviewed

European Projects :

FP7 - 201370 - LUPA - Unravelling the molecular basis of common complex human disorders using the dog as a model system

Funders :

CE - Commission Européenne

Available on ORBi :

since 07 August 2018

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