Reference : pH and Not Cell Morphology Modulate pLIP2 Induction in the Dimorphic Yeast Yarrowia l...
Scientific journals : Letter to the editor
Life sciences : Biotechnology
http://hdl.handle.net/2268/226166
pH and Not Cell Morphology Modulate pLIP2 Induction in the Dimorphic Yeast Yarrowia lipolytica
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Sassi, Hosni mailto [Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies >]
Delvigne, Frank mailto [Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies >]
Kallel, H. [Laboratory of Molecular Microbiology, Vaccinology and Biotechnology Development, Biofermentation Unit, Institut Pasteur de Tunis, Université Tunis El Manar, Tunis, Tunisia]
Fickers, Patrick mailto [Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies >]
2017
Current Microbiology
Springer New York LLC
74
3
413-417
Yes (verified by ORBi)
0343-8651
1432-0991
[en] Letter ; LIP2 gene ; Yarrowia lipolytica ; Yarrowia ; Fungal Proteins ; Gene Expression Regulation, Fungal ; Hydrogen-Ion Concentration ; Recombinant Proteins ; Yarrowia
[en] The dimorphic yeast Yarrowia lipolytica has become an emerging cell factory for recombinant proteins production. Expression vectors involving LIP2 promoter (pLIP2) have been developed and used successfully. However, the relationship between dimorphic transition (i.e., cell morphology) and pLIP2 regulation is still unclear and must be assessed to improve process robustness. This requests to discriminate the effect of cell morphology from that of effectors, such as pH, that trigger the dimorphic transition. This was performed using gene reporter system based on β-galactosidase activity and DsRed fluorescence, single-cell analysis by flow cytometry, and quantification of gene expression. Our results clearly pointed out that cell morphology has not effect on the regulation of pLIP2. By contrast, pH modification yielded to phenotypic heterogeneity, potentially leading to a lack of robustness of the cell population. Taken altogether, our results demonstrated that, under appropriate environmental conditions (e.g., pH being an important factor), Y. lipolytica could be considered as a robust and reliable host for recombinant protein production. © 2017, Springer Science+Business Media New York.
http://hdl.handle.net/2268/226166
10.1007/s00284-017-1207-0

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