Sulphated polysaccharide isolated from Sargassum vulgare: Characterization and hypolipidemic effects

Dyslipidemia; GCMS analysis; NMR spectroscopy; Sargassum vulgare; Sulphated polysaccharide; Algae; Chromatography; Differential scanning calorimetry; Lipids; Mass spectrometry; Nuclear magnetic resonance spectroscopy; Spectroscopic analysis; Toxicity; X ray diffraction analysis; Dyslipidemias; Gas chromatography-mass spectrometry; GC-MS analysis; Homogeneous structure; Semi-crystalline polymer; Structural characterization; Sulphated polysaccharides; Gas chromatography

Abstract :

[en] A sulphated polysaccharide from brown algae Sargassum vulgare (SVSP) was extracted and examined with respect to chemical, structural characterization and hypolipidemic effects. SVSP consisted mainly of sulphate and total sugars with low levels of lipids and proteins. Its structure was studied by nuclear magnetic resonance (RMN), gas chromatography-mass spectrometry (GC–MS), infra-red spectroscopic, differential scanning calorimetry and X-ray diffraction analysis. Allowing us therefore to revealed that SVSP was composed of glucose, rhamnose, xylose, galactose, mannose and arabinose with XRD pattern that was typical for a semi-crystalline polymer and complexities of the spectra reflected its homogeneous structure. The administration of SVSP to obese rats is effective in lowering the body weight and inhibiting the lipase activity leading to notable regulation of lipid profile, increasing the activities of antioxidant enzymes, limiting lipid peroxidation; and protects liver-kidney functions proved by a decrease in the levels of toxicity parameters in blood, confirmed by histological study. © 2017

Disciplines :

Food science

Author, co-author :

Kolsi, R. B. A.; Laboratory of Plant Biotechnology, Faculty of Sciences of Sfax, Sfax, Tunisia

Salah, H. B.; Laboratory of chemistry of Natural Substances, Faculty of Sciences of Sfax, PB 802, Sfax, Tunisia

Jardak, N.; Research Unit of anatomy, histology and embryology, Faculty of Medicine Sfax, Tunisia

Chaaben, R.; Biochemistry Laboratory, CHU Hedi Chaker, Sfax, Tunisia

Jribi, I.; Biodiversity Unit and Aquatic Ecosystems, Faculty of Sciences of Sfax, Sfax, Tunisia

Feki, A. E.; Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax, Tunisia

Rebai, T.; Research Unit of anatomy, histology and embryology, Faculty of Medicine Sfax, Tunisia

Jamoussi, K.; Biochemistry Laboratory, CHU Hedi Chaker, Sfax, Tunisia

Allouche, N.; Laboratory of chemistry of Natural Substances, Faculty of Sciences of Sfax, PB 802, Sfax, Tunisia

Blecker, Christophe ; Université de Liège - ULiège > SMARTECH

Belghith, H.; Enzyme and Bioconversion Unit, Biotechnology Center of Sfax, Tunisia

Belghith, K.; Laboratory of Plant Biotechnology, Faculty of Sciences of Sfax, Sfax, Tunisia

Language :

English

Title :

Sulphated polysaccharide isolated from Sargassum vulgare: Characterization and hypolipidemic effects

Publication date :

2017

Journal title :

Carbohydrate Polymers

ISSN :

0144-8617

eISSN :

1879-1344

Publisher :

Elsevier Ltd

Volume :

170

Pages :

148-159

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 12 June 2018

Statistics

Number of views

64 (1 by ULiège)

Number of downloads

0 (0 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

Bibliography

Aebi, H., Catalase in vitro. Methods in Enzymology 105 (1984), 121–126.
Alban, S., Schauerte, A., Franz, G., Anticoagulant sulfated polysaccharides: Part I. Synthesis and structure-activity relationships of new pullulan sulfates. Carbohydrate Polymers 47 (2002), 267–276.
Ananthi, S., Raghavendran, H.R.B., Sunil, A.G., Gayathri, V., Ramakrishnan, G., Vasanthi, H.R., In vitro antioxidant and in vivo anti-inflammatory potential of crude polysaccharide from Turbinaria ornata (Marine Brown Alga). Food and Chemical Toxicology 48:1 (2010), 187–192.
Aoac, W.H., Official methods of analysis of the Association of Official Analytical Chemists. 1990, Association of Official Analytical Chemists, Arlington, VA, USA.
Arechederra, R.L., Waheed, A., Sly, W.S., Supuran, C.T., Minteer, S.D., Effect of sulfonamides as carbonic anhydrase VA and VB inhibitors on mitochondrial metabolic energy conversion. Bioorganic & Medicinal Chemistry 21:6 (2013), 1544–1548.
Attia, D.M., Ni, Z.N., Boer, P., Attia, M.A., Goldshmeding, R., Koomans, H.A., et al. Proteinuria is preceded by decreased nitric oxide synthesis and prevented by a NO donor in cholesterol-fed rats. Kidney International 61 (2002), 1776–1787.
Bays, H.E., Gadde, K.M., Phentermine/topiramate for weight reduction and treatment of adverse metabolic consequences in obesity. Drugs of Today (Barcelona Spain: 1998) 47:12 (2011), 903–914.
Bentley, M.D., Rodriguez-Porcel, M., Lerman, A., Sarafov, M.H., Romero, J.C., Pelaez, L.I., et al. Enhanced renal cortical vascularization in experimental hypercholesterolemia. Kidney In 61 (2002), 1056–1063.
Birari, R., Javia, V., Bhutani, K.K., Antiobesity and lipid lowering effects of Murraya koenigii (L.) Spreng leaves extracts and mahanimbine on high fat diet induced obese rats. Fitoterapia 81:8 (2010), 1129–1133.
Bitter, T., Muir, H.M., A modified uronic acid carbazole reaction. Analytical Biochemistry 4:4 (1962), 330–334.
Bligh, E.G., Dyer, W.J., A rapid method of total lipid extraction andpurification. Canadian Journal of Biochemistry and Physiology 37:8 (1959), 911–917.
Buege, J.A., Aust, S.D., [30] microsomal lipid peroxidation. Methods in Enzymology 52 (1978), 302–310.
De Barros, R.P., Measuring inequality of opportunities in Latin America and the Caribbean. 2009, World Bank Publications.
Dietrich, C.P., Farias, G.G., de Abreu, L.R., Leite, E.L., da Silva, L.F., Nader, H.B., A new approach for the characterization of polysaccharides from algae: Presence of four main acidic polysaccharides in three species of the class Phaeophycea. Plant Science 108:2 (1995), 143–153.
Dore, C.M.P.G., Alves, M.G.D.C.F., Will, L.S.E.P., Costa, T.G., Sabry, D.A., de Souza Rêgo, L.A.R., et al. A sulfated polysaccharide, fucans, isolated from brown algae Sargassum vulgare with anticoagulant, antithrombotic, antioxidant and anti-inflammatory effects. Carbohydrate Polymers 91:1 (2013), 467–475.
El Gerssifi, M., Les défauts des produits de patisserie et biscuiterie au coursdu stockage: La prévention par la formulation. Industries Alimentaires EtAgricoles 115:7–8 (1998), 82–88.
Figueiro, S.D., Macêdo, A.A.M., Melo, M.R.S., Freitas, A.L.P., Moreira, R.A., De Oliveira, R.S., et al. On the dielectric behaviour of collagen–algal sulfated polysaccharide blends: effect of glutaraldehyde crosslinking. Biophysical Chemistry 120:2 (2006), 154–159.
Gonca, S., Ceylan, S., Yardimoglu, M., Dalcik, H., Yumbul, Z., Kokturk, S., et al. Protective effects of vitamin E and selenium on the renal morphology in rats fed high-cholesterol diets. Pathobiology 68 (2000), 258–263.
Halpern, A., Mancini, M.C., Treatment of obesity: An update on anti-obesity medications. Obesity Reviews 4 (2003), 25–42.
Hassan, S., El-Twab, S.A., Hetta, M., Mahmoud, B., Improvement of lipid profile and antioxidant of hypercholesterolemic albino rats by polysaccharides extracted from the green alga Ulva lactuca Linnaeus. Saudi Journal of Biological Sciences 18:4 (2011), 333–340.
Hedge, J.E., Hofreiter, B.T., Whistler, R.L., Be Miller, J.N., Carbohydratechemistry. 1962, Academic Press, New York, 17.
Huang, L., Wen, K., Gao, X., Liu, Y., Hypolipidemic effect of fucoidan from Laminaria japonica in hyperlipidemic rats. Pharmaceutical Biology 48:4 (2010), 422–426.
Jallabert, B. (2014). Etude du comportement sous pression mécanique uniaxiale dela cellulose et de l'amidon (natif et amorphe): influence de la température etdu taux d'hydratation.
Jones, R., Mann, T., Sherins, R., Peroxidative breakdown of phospholipids in human spermatozoa: spermicidal properties of fatty acid peroxides and protective action of seminal plasma. Fertility and Sterility 31 (1979), 531–537.
Karaki, N., Sebaaly, C., Chahine, N., Faour, T., Zinchenko, A., Rachid, S., et al. The antioxidant and anticoagulant activities of polysaccharides isolated from the brown algae Dictyopteris polypodioides growing on the Lebanese coast. Journal of Applied Pharmaceutical Science, 3(2), 2013, 43.
Karnjanapratum, S., Tabarsa, M., Cho, M., You, S., Characterization and immunomodulatory activities of sulfated polysaccharides from Capsosiphon fulvescens. International Journal of Biological Macromolecules 51:5 (2012), 720–729.
Kesari, A.N., Kesari, S., Singh, S.K., Gupta, R.K., Watal, G., Studies on the glycemic and lipidemic effect of Murraya koenigii in experimental animals. Journal of Ethnopharmacology 112 (2007), 305–311.
Krichen, F., Karoud, W., Sila, A.D., Abdelmalek, B.E., Ghorbel, R., Ellouz-Chaabouni, S., et al. Extraction: characterization and antimicrobial activity ofsulfated polysaccharides from fish skins. International Journal of Biological Macromolecules 75 (2015), 283–289.
Lahaye, M., Axelos, M.A.V., Gelling properties of water-solublepolysaccharides from proliferating marine green seaweeds (Ulva spp.). Carbohydrate Polymers 22:4 (1993), 261–265.
Lange III, L. G., & Spilburg, C. A. (1991). U.S. Patent No. 5, 017, 565. Washington, DC: U.S. Patent and Trademark Office.
Leite, E.L., Medeiros, M.G., Rocha, H.A., Farias, G.G., da Silva, L.F., Chavante, S.F., et al. Structure and pharmacological activities of a sulfated xylofucoglucuronan from the alga Spatoglossum schröederi. Plant Science 132:2 (1998), 215–228.
Lemoine, M., Helbert, W., Dégradation enzymatique en phase hétérogénedes polysaccharides: exemple des agarases et des carraghénases. Journal De LaSociété De Biologie 201:3 (2007), 291–296.
Lissner, L., Heitmann, B.L., Dietary fat and obesity: Evidence from epidemiology. European Journal of Clinical Nutrition 49 (1995), 79–90.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry 193:1 (1951), 265–275.
Mah, P.M., Wittert, G.A., Obesity and testicular function. Molecular and Cellular Endocrinology 316:2 (2010), 180–186.
Marinho-Soriano, E., Agar polysaccharides from gracilaria species(Rhodophyta, gracilariaceae). Journal of Biotechnology 89:1 (2001), 81–84.
Marklund, S., Marklund, G., Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry 47:3 (1974), 469–474.
Mori, Y., Hirano, T., Ezetimibe alone or in combination with pitavastatin prevents kidney dysfunction in 5/6 nephrectomized rats fed high-cholesterol. Metabolism 61 (2012), 379–388.
Ortiz, J., Romero, N., Robert, P., Araya, J., Lopez-Hernandez, J., Bozzo, C., et al. Dietary fiber amino acid, fatty acid and tocopherol contents of theedible seaweeds Ulva lactuca and Durvillaea antarctica. Food Chemistry 99:1 (2006), 98–104.
Padron, R.S., Mas, J., Zamora, R., Riverol, F., Licea, M., Mallea, L., et al. Lipids and testicular function. International Urology and Nephrology 21 (1989), 515–519.
Paglia, D.E., Valentine, W.N., Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal of Laboratory and Clinical Medicine 70:1 (1967), 158–169.
Peng, Z., Liu, M., Fang, Z., Wu, J., Zhang, Q., Composition and cytotoxicity of a novel polysaccharide from brown alga (Laminaria japonica). Carbohydrate Polymers 89:4 (2012), 1022–1026.
Qi, H., Zhang, Q., Zhao, T., Chen, R., Zhang, H., Niu, X., et al. Antioxidant activity of different sulfate content derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta) in vitro. International Journal of Biological Macromolecules 37:4 (2005), 195–199.
Raghavendran, H.R.B., Sathivel, A., Devaki, T., Effect of Sargassum polycystum (Phaeophyceae)-sulphated polysaccharide extract against acetaminophen-induced hyperlipidemia during toxic hepatitis in experimental rats. Molecular and Cellular Biochemistry 276:1-2 (2005), 89–96.
Rahman, M.S., State diagram of date flesh using differential scanning calorimetry (DSC). International Journal of Food Properties 7 (2004), 407–428.
Rioux, L.E., Turgeon, S.L., Beaulieu, M., Characterization ofpolysaccharides extracted from brown seaweeds. Carbohydrate Polymers 69:3 (2007), 530–537.
Robic, A., Gaillard, C.D., Sassi, J.F.O., Lerat, Y., Lahaye, M., Ultrastructureof ulvan: a polysaccharide from green seaweeds. Biopolymers 91:8 (2009), 652–664.
Ronkart, S.B., Blecker, C.S., Deroanne, C., Paquot, M., Phénoméne de latransition vitreuse appliquée aux glucides alimentaires amorphes à l’état depoudre/Glass transition phenomena applied to powdered amorphous foodcarbohydrates Biotechnologie. Agronomie. Société Et Environnement, 13(1), 2009, 177.
Sathivel, A., Raghavendran, H.R.B., Srinivasan, P., Devaki, T., Anti-peroxidative and anti-hyperlipidemic nature of Ulva lactuca crude polysaccharide on D-galactosamine induced hepatitis in rats. Food and Chemical Toxicology 46:10 (2008), 3262–3267.
Shalaby, M.A., El Zorba, H.Y., Kamel, G.M., Effect of α-tocopherol and simvastatin on male fertility in hypercholesterolemic rats. Pharmacological Research 50:2 (2004), 137–142.
Slim, R.M., Toborek, M., Watkins, B.A., Boissonneault, G.A., Hennig, B., Susceptibility to hepatic oxidative stress in rabbits fed different animal and plant fats. Journal of the American College of Nutrition 15:3 (1996), 289–294.
Spěváček, J., Brus, J., Solid-State NMR studies of polysaccharide systems. Macromolecular Symposia 265:May (1) (2008), 69–76 [WILEYčVCH Verlag].
Sudhop, T., Lütjohann, D., Kodal, A., Igel, M., Tribble, D.L., Shah, S., et al. Inhibition on intestinal cholesterol absorption by ezetimibe in humans. Circulation 106 (2002), 1943–1948.
Vasconcelos, M.T.S., Leal, M.F.C., Seasonal variability in the kinetics of Cu, Pb, Cd and Hg accumulation by macroalgae. Marine Chemistry 74:1 (2001), 65–85.
Wang, Y., Peng, Y., Wei, X., Yang, Z., Xiao, J., Jin, Z., Sulfation of teapolysaccharides: synthesis, characterization and hypoglycemic activity. International Journal of Biological Macromolecules 46:2 (2010), 270–274.
Wang, X., Zhang, Z., Yao, Z., Zhao, M., Qi, H., Sulfation: anticoagulant andantioxidant activities of polysaccharide from green algae Enteromorpha linza. International Journal of Biological Macromolecules. 58 (2013), 225–230.
Xiong, S.L., Li, A.L., Jin, Z.Y., Chen, M., Effects of oral chondroitin sulfate on lipid and antioxidant metabolisms in rats fed a high‐fat diet. Journal of Food Biochemistry 31 (2007), 356–369.
Yaich, H., Garna, H., Bchir, B., Besbes, S., Paquot, M., Richel, A., et al. Chemical composition and functional properties of dietary fibre extracted byEnglyst and Prosky methods from the alga Ulva lactuca collected in Tunisia. Algal Research 9 (2015), 65–73.