Doctoral thesis (Dissertations and theses)
Caractérisation structurale et fonctionnelle du domaine de fixation à la chitine de la chitotriosidase de macrophage humain
Crasson, Oscar
2018
 

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Keywords :
Chitotriosidase; Carbohydrate Binding Module; Chitine
Abstract :
[en] Chitin is an important structural component of cell walls and exoskeletons of numerous organisms. Chitin binding proteins are closely related to those structures. They are associated to chitin stability, remodelling, recognition, metabolism and tens of biological functions. ChBD, the chitin binding domain of the human chitotriosidase (HCHT) attracted our attention due to a considerable lack of information regarding this domain. Based on literature, CBMs are known to influence significantly the behaviour of their cognate catalytic domains. We wanted to verify if it was the case for ChBD and to determine in which manner this phenomenon could occur. We investigated the biochemical function of ChBD by chitin binding studies. We analysed the structure of the CBM and defined a binding mechanism in accordance to previous publications and our experimental data. A phylogenetic analysis was used to determine the evolution path of HCHT and its constitutive domains. Altogether, our results make the link between chitin, innate immunity, ChBD and HTCH appearance.
Research center :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Crasson, Oscar ;  Université de Liège - ULiège > Doct. sc. (bioch., biol. mol.&cell., bioinf.&mod.-Bologne)
Language :
French
Title :
Caractérisation structurale et fonctionnelle du domaine de fixation à la chitine de la chitotriosidase de macrophage humain
Alternative titles :
[en] Structural and functional characterization of the chitin binding domain from the human macrophage chitotriosidase
Defense date :
30 May 2018
Number of pages :
183
Institution :
ULiège - Université de Liège
Degree :
Docteur en Biochimie, Biologie Moléculaire et Cellulaire, Bioinformatique et Modélisation
Promotor :
Galleni, Moreno ;  Université de Liège - ULiège > Integrative Biological Sciences (InBioS)
Coutinho, Pedro
President :
Damblon, Christian ;  Université de Liège - ULiège > Molecular Systems (MolSys)
Secretary :
Dumoulin, Mireille  ;  Université de Liège - ULiège > Integrative Biological Sciences (InBioS)
Jury member :
Terrak, Mohammed  ;  Université de Liège - ULiège > Integrative Biological Sciences (InBioS)
Vandenbussche, Guy
Name of the research project :
Caractérisation structurale et fonctionnelle du domaine de fixation à la chitine de la chitotriosidase de macrophage humain
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
Available on ORBi :
since 30 May 2018

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