Reference : Investigation of solid surface-enhanced Raman scattering (SERS) substrates for the qu...
Scientific congresses and symposiums : Poster
Physical, chemical, mathematical & earth Sciences : Chemistry
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/223512
Investigation of solid surface-enhanced Raman scattering (SERS) substrates for the quantification of dopamine in cell culture media
English
Dumont, Elodie mailto [Université de Liège - ULiège > Département de pharmacie > Chimie analytique >]
De Bleye, Charlotte mailto [Université de Liège - ULiège > Département de pharmacie > Département de pharmacie >]
Cailletaud, Johan mailto [Université de Liège - ULiège > Département de pharmacie > Chimie analytique >]
Deidda, Riccardo mailto [Université de Liège - ULiège > Département de pharmacie > Chimie analytique >]
Sacre, Pierre-Yves mailto [Université de Liège - ULiège > Département de pharmacie > Chimie analytique >]
Hubert, Philippe mailto [Université de Liège - ULiège > Département de pharmacie > Chimie analytique >]
Ziemons, Eric mailto [Université de Liège - ULiège > Département de pharmacie > Département de pharmacie >]
17-May-2018
No
No
National
Bioforum of BioLiège 2018
17 mai 2018
BioLiège
Ecole doctorale de Biologie Cellulaire et Moléculaire, Biochimie
Ecole doctorale de Structure and function of biological macromolecules, bioinformatics and modelling
Ecole doctorale Plant Science
Ecole doctorale Biodiversity - Ecology - Evolution
Ecole doctorale de Sciences Pharmaceutiques
Ecole doctorale de Cancérologie Expérimentale
Ecole doctorale Veterinary Sciences
Liège
Belgium
[en] Surface-Enhanced Raman Scattering (SERS) ; Gold and Silver Nanoparticles ; Solid SERS Substrates ; Dopamine ; Quantitative methods ; PC-12 cells
[en] Since its discovery, surface-enhanced Raman spectroscopy (SERS) has constantly gained ground among the analytical techniques. One field of SERS that is rapidly expanding concerns the development of solid SERS substrates. These are made up of metallic nanomaterials set in a fixed configuration: either by complex and expensive techniques (lithography, printing) or by the immobilisation of suspensions of nanoparticles (NPs) previously synthesised. In bioanalysis, solid SERS substrates allow the performance of real-time monitoring of biological or cellular processes such as the release of neurotransmitters by stimulated cells or intercellular communications between cancer cells and the immune system. Dopamine (DA) is an important neurotransmitter of the central and peripheral nervous systems, as evidenced by the diseases arising when DA levels are dysregulated. Consequently, analytical techniques being able to determine DA in sensitive and specific ways are requested. In this context, this work was dedicated to the development of solid SERS substrates enabling the quantification of DA in the culture medium of rat phaeochromocytoma PC-12 cells. These cells are a cellular model for the neurosecretion of DA. The solid SERS substrates developed could furthermore be employed to investigate the cellular release of DA under various stimulation conditions directly in the culture medium.
Centre Interdisciplinaire de Recherche sur le Médicament - CIRM
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/223512

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