Reference : Development of a SERS strategy to overcome the nanoparticle stabilisation effect in s...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/222054
Development of a SERS strategy to overcome the nanoparticle stabilisation effect in serum-containing samples: Application to the quantification of dopamine in the culture medium of PC-12 cells
English
Dumont, Elodie mailto [Université de Liège - ULiège > Département de pharmacie > Chimie analytique >]
De Bleye, Charlotte mailto [Université de Liège - ULiège > Département de pharmacie > Département de pharmacie >]
Cailletaud, Johan mailto [Université de Liège - ULiège > Département de pharmacie > Chimie analytique >]
Sacre, Pierre-Yves mailto [Université de Liège - ULiège > Département de pharmacie > Chimie analytique >]
Van Lerberghe, Pierre-Bernard mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Rogister, Bernard mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine >]
Rance, Graham A mailto [University of Nottingham > > Nanoscale and Microscale Research Centre > >]
Aylott, Jonathan W mailto [University of Nottingham > Pharmacy > Laboratory of Biophysics and Surface Analysis > >]
Hubert, Philippe mailto [Université de Liège - ULiège > Département de pharmacie > Chimie analytique >]
Ziemons, Eric mailto [Université de Liège - ULiège > Département de pharmacie > Département de pharmacie >]
15-Aug-2018
Talanta
Elsevier
186
8-16
Yes (verified by ORBi)
International
0039-9140
1873-3573
Netherlands
[en] Surface-Enhanced Raman Scattering (SERS) ; Gold Nanoparticles ; Dopamine ; Quantitative methods ; Cellular analysis ; PC-12 cells
[en] The analysis of serum samples by surface-enhanced Raman spectroscopy (SERS) has gained ground over the last years. However, the stabilisation of colloids by the proteins contained in these samples has restricted their use in common practice, unless antibodies or aptamers are used. Therefore, this work was dedicated to the development of a SERS methodology allowing the analysis of serum samples in a simple and easy-to-implement way. This approach was based on the pre-aggregation of the colloid with a salt solution. Gold nanoparticles (AuNPs) were used as the SERS substrate and, owing to its physiopathological importance, dopamine was chosen as a model to implement the SERS approach. The presence of this neurotransmitter could be determined in the concentration range 0.5 to 50 ppm (2.64 – 264 µM) in the culture medium of PC-12 cells, with a R² of 0.9874, and even at lower concentrations (0.25 ppm, 1.32 µM) in another matrix containing fewer proteins. Moreover, the effect of calcium and potassium on the dopamine exocytosis from PC-12 cells was studied. Calcium was shown to have a predominant and dose-dependent effect. Finally, PC-12 cells were exposed to dexamethasone in order to increase their biosynthesis and release of dopamine. This increase was monitored with the developed SERS approach.
Centre Interdisciplinaire de Recherche sur le Médicament - CIRM
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/222054
10.1016/j.talanta.2018.04.038

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