Article (Scientific journals)
Volatile fingerprinting of human respiratory viruses from cell culture
Purcaro, Giorgia; Rees, Chris; Wieland-Alter, Wendy et al.
2017In Journal of Breath Research, 12 (2)
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Keywords :
Virus; VOCs; GCxGC
Abstract :
[en] Volatile metabolites are currently under investigation as potential biomarkers for the detection and identification of pathogenic microorganisms, including bacteria, fungi, and viruses. Unlike bacteria and fungi, which produce distinct volatile metabolic signatures associated with innate differences in both primary and secondary metabolic processes, viruses are wholly reliant on the metabolic machinery of infected cells for replication and propagation. In the present study, the ability of volatile metabolites to discriminate between respiratory cells infected and uninfected with virus, in vitro, was investigated. Two important respiratory viruses, namely respiratory syncytial virus (RSV) and influenza A virus (IAV), were evaluated. Data were analyzed using three different machine learning algorithms (random forest (RF), linear support vector machines (linear SVM), and partial least squares-discriminant analysis (PLS-DA)), with volatile metabolites identified from a training set used to predict sample classifications in a validation set. The discriminatory performances of RF, linear SVM, and PLS-DA were comparable for the comparison of IAV-infected versus uninfected cells, with area under the receiver operating characteristic curves (AUROCs) between 0.78 and 0.82, while RF and linear SVM demonstrated superior performance in the classification of RSV-infected versus uninfected cells (AUROCs between 0.80 and 0.84) relative to PLS-DA (0.61). A subset of discriminatory features were assigned putative compound identifications, with an overabundance of hydrocarbons observed in both RSV- and IAV-infected cell cultures relative to uninfected controls. This finding is consistent with increased oxidative stress, a process associated with viral infection of respiratory cells.
Disciplines :
Chemistry
Author, co-author :
Purcaro, Giorgia  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Rees, Chris
Wieland-Alter, Wendy
Schneider, Mark
Wang, Xi
Stefanuto, Pierre-Hugues  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique, organique et biologique
Wright, Peter
Enelow, Richard
Hill, Jane
Language :
English
Title :
Volatile fingerprinting of human respiratory viruses from cell culture
Publication date :
December 2017
Journal title :
Journal of Breath Research
ISSN :
1752-7155
eISSN :
1752-7163
Publisher :
Institute of Physics (IOP), Bristol, United Kingdom
Volume :
12
Issue :
2
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
NIH - National Institutes of Health [US-MD]
Available on ORBi :
since 15 March 2018

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