Article (Scientific journals)
A fast and simple method for simultaneous measurements of 25(OH)D, 24,25(OH)2D and the Vitamin D Metabolite Ratio (VMR) in serum samples by LC-MS/MS
Fabregat Cabello, Neus; Farre Segura, Jordi; Huyghebaert, Loreen et al.
2017In Clinica Chimica Acta
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Keywords :
Vitamin D metabolite ratio; Liquid Chromatography; Mass spectrometry
Abstract :
[en] BACKGROUND: Rapid, easy and reliable measurement of the major vitamin D metabolites is required in order to fulfill the needs of a clinical routine laboratory. To overcome these challenges, we have developed and validated a LC-MS/MS method for the quantification of 25-hydroxyvitamin D2 and D3, epi-25-hydroxyvitamin D3 and 24,25-dihydroxyvitamin D3. METHODS: Sample preparation was based on precipitation and centrifugation of 100 µL of patient serum, followed by injection into the LC-MS/MS system. Samples from Vitamin D Standardization Program (n=80) and patient samples (n=281) have been compared with a reference LC-MS/MS method. For the analytical validation NIST and Labquality quality control materials were used. RESULTS: Mean intra-assay and inter-assay imprecision were less than 6.0 and 6.4% and mean recoveries were within 95-104%. LOQ’s were 0.5 µg/L for 24,25(OH)2D3, 1.1 µg/L for 25(OH)D3 and epi-25(OH)D3 and 1.7 µg/L for 25(OH)D2. A 3% bias obtained between the proposed and the reference method satisfies Vitamin D Standardization Program recommendations. CONCLUSIONS: We present a rapid, easy, reliable and cost-effective method completely adequate for routine testing, which permits the measurement of the ratio of 24,25-dihydroxyvitamin D to 25-hydroxyvitamin D, Vitamin D Metabolite Ratio (VMR), in serum samples.
Disciplines :
Endocrinology, metabolism & nutrition
Author, co-author :
Fabregat Cabello, Neus ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Farre Segura, Jordi  ;  Université de Liège - ULiège > conv. Erasmus en médecine
Huyghebaert, Loreen ;  Centre Hospitalier Universitaire de Liège - CHU > Service de chimie clinique
PEETERS, Stéphanie ;  Centre Hospitalier Universitaire de Liège - CHU > Service de chimie clinique
LE GOFF, Caroline  ;  Centre Hospitalier Universitaire de Liège - CHU > Service de chimie clinique
Souberbielle, Jean-Claude;  Laboratoire d’Exploitations fonctionnelles, > Hôpital Necker-Enfants malades, Paris,
Cavalier, Etienne  ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Language :
English
Title :
A fast and simple method for simultaneous measurements of 25(OH)D, 24,25(OH)2D and the Vitamin D Metabolite Ratio (VMR) in serum samples by LC-MS/MS
Publication date :
August 2017
Journal title :
Clinica Chimica Acta
ISSN :
0009-8981
eISSN :
1873-3492
Publisher :
Elsevier Science, Amsterdam, Netherlands
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 18 September 2017

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