Lc-chip versus UHPLC-tandem mass spectrometry for the quantitation of estetrol and estradiol without derivatization after whole blood microsampling

the aim of this work was to conduct a PK study on mice to select the most appropriate administration route for E2 and E4 formulations. To achieve this goal, a reference method for the quantitation of both estrogens after whole blood microsampling was developed and validated on a UHPLC-MS/MS system. This reference method was later transferred on LC-chip device and both methods were compared in terms of analytical parameters such as response function, accuracy, precision, trueness and limit of quantification.