[en] We report here on EYK1, encoding erythrulose kinase, as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Compared to auxotrophic markers, EYK1 increases the growth rate of transformants and allows improved efficiency of transformation. The utility of the marker EYK1 in a replicative vector was also demonstrated.
Disciplines :
Biotechnology
Author, co-author :
Vandermies, Marie ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Denies, Olivia ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Nicaud, Jean-Marc
Fickers, Patrick ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Language :
English
Title :
EYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing in the non-conventional yeast Yarrowia lipolytica
Publication date :
August 2017
Journal title :
Journal of Microbiological Methods
ISSN :
0167-7012
eISSN :
1872-8359
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
139
Pages :
161-164
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
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