EYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing in the non-conventional yeast Yarrowia lipolytica

Vandermies, Marie; Denies, Olivia; Nicaud, Jean-Marc; Fickers, Patrick

doi:10.1016/j.mimet.2017.05.012

Article (Scientific journals)

EYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing in the non-conventional yeast Yarrowia lipolytica

Vandermies, Marie; Denies, Olivia; Nicaud, Jean-Marc et al.

2017 • In Journal of Microbiological Methods, 139, p. 161-164

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/212994

DOI
10.1016/j.mimet.2017.05.012

PubMed
28552427

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Keywords :

Yarrowia lipolytica; Genome editing; EYK1; Catabolic selectable marker

Abstract :

[en] We report here on EYK1, encoding erythrulose kinase, as an eﬃcient catabolic selectable marker for genome editing in Y. lipolytica. Compared to auxotrophic markers, EYK1 increases the growth rate of transformants and allows improved eﬃciency of transformation. The utility of the marker EYK1 in a replicative vector was also demonstrated.

Disciplines :

Biotechnology

Author, co-author :

Vandermies, Marie ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies

Denies, Olivia ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies

Nicaud, Jean-Marc

Fickers, Patrick ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies

Language :

English

Title :

EYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing in the non-conventional yeast Yarrowia lipolytica

Publication date :

August 2017

Journal title :

Journal of Microbiological Methods

ISSN :

0167-7012

eISSN :

1872-8359

Publisher :

Elsevier Science, Amsterdam, Netherlands

Volume :

139

Pages :

161-164

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture

Available on ORBi :

since 17 July 2017

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Bibliography

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