Reference : Streptomyces from Moonmilk, an Untapped Source of New Bioactive Compounds
Scientific congresses and symposiums : Poster
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/212817
Streptomyces from Moonmilk, an Untapped Source of New Bioactive Compounds
English
Martinet, Loïc mailto [Université de Liège > Département des sciences de la vie > Département des sciences de la vie >]
Maciejewska, Marta []
Adam, Delphine mailto [Université de Liège > Département des sciences de la vie > Département des sciences de la vie >]
Naomé, Aymeric mailto [Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines >]
Carnol, Monique mailto [Université de Liège > Département de Biologie, Ecologie et Evolution > Ecologie végétale et microbienne >]
Barton, Hazel A. []
Smargiasso, Nicolas mailto [Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.) >]
De Pauw, Edwin mailto [Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.) >]
Hanikenne, Marc mailto [Université de Liège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale >]
Baurain, Denis mailto [Université de Liège > Département des sciences de la vie > Phylogénomique des eucaryotes >]
Rigali, Sébastien mailto [Université de Liège > Département des sciences de la vie > Département des sciences de la vie >]
24-May-2017
ArcheE
No
Yes
International
International symposium on the biology of Actinomycetes
du 23 au 27 mai 2017
Jeju
Corée du Sud
[en] Streptomyces lunaelactis ; PKS, NRPS ; antibiotics
[en] Moonmilk speleothems of limestone caves host a rich microbiome, among which Actinobacteria represent one of the most abundant phyla. Ancient medical texts reported that moonmilk had therapeutical properties, thereby suggesting that its filamentous endemic actinobacterial population might be a source of natural products useful in human treatment. In this work, a screening approach was undertaken in order to isolate cultivable Actinobacteria from moonmilk of the Grotte des Collemboles in Belgium, to evaluate their taxonomic profile, and to assess their potential in biosynthesis of antimicrobials. Phylogenetic analysis revealed that all 78 isolates were exclusively affiliated to the genus Streptomyces and clustered into 31 distinct phylotypes displaying various pigmentation patterns and morphological features. Phylotype representatives were tested for antibacterial and antifungal activities and their genomes were mined for secondary metabolite biosynthetic genes coding for non-ribosomal peptide synthetases (NRPSs), and polyketide synthases (PKS). The moonmilk Streptomyces collection was found to display strong inhibitory activities against a wide range of reference organisms, as 94, 71, and 94% of the isolates inhibited or impaired the growth of Gram-positive, Gram-negative bacteria, and fungi, respectively. Interestingly, 90% of the cave strains induced strong growth suppression against the multi-drug resistant Rasamsonia argillacea, a causative agent of invasive mycosis in cystic fibrosis and chronic granulomatous diseases. We are currently identifying molecules responsible for the observed antimicrobial activities which, next to few known bioactive compounds, also reveals many active compounds with molecular masses that do not correspond to known antibiotic deposited in web biomolecules databases such as KNapSAcK, Pubchem, Chemspider. The challenge is to increase the production yields of these unknown compounds, and purify them to further characterize their structure by NMR. Overall, our work supports the common belief that moonmilk might effectively treat various infectious diseases thanks to the presence of a highly diverse population of prolific antimicrobial producing Streptomyces, and thus may indeed constitute a promising reservoir of potentially novel active natural compounds.
Biological Sciences from Molecules to Systems - inBioS ; Centre d'Ingénierie des Protéines - CIP
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; subside facultaire pour colloque-congrès
Carractérisation du métabolisme secondaire des Streptomyces d'origine karstique
Researchers ; Professionals ; Students ; General public
http://hdl.handle.net/2268/212817

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