[en] Microbiome studies generally focus on the gut microbiome, which is composed of a large proportion of commensal bacteria. Here we propose a first analysis of the liver microbiomeusing next generation sequencing as a tool to detect potentially pathogenic strains. We used Peromyscus leucopus, the main reservoir host species of Lyme disease in eastern North America, as a model and sequenced V5-V6 regions of the 16S gene from 18 populations in southern Quebec (Canada). The Lactobacillus genus was found to dominate the liver microbiome.We also detected a large proportion of individuals infected by Bartonella vinsonii arupensis, a human pathogenic bacteria responsible for endocarditis, aswell as Borrelia burgdorferi, the pathogen responsible for Lyme disease in North America. We then compared the microbiomes among two P. leucopus genetic clusters occurring on either side of the St. Lawrence River, and did not detect any effect of the host genotype on their liver microbiome assemblage. Finally, we report, for the first time, the presence of B. burgdorferi in a smallmammal host fromthe northern side of the St. Lawrence River, in support of models that have predicted the northern spread of Lyme disease in Canada.
Disciplines :
Microbiology
Author, co-author :
André, Adrien ; Université de Liège > Département des sciences de la vie > Génétique et physiologie des microalgues
Mouton, Alice ; Université de Liège > Département des sciences de la vie > Génétique et physiologie des microalgues
millien, virginie
Michaux, Johan ; Université de Liège > Département des sciences de la vie > Génétique et physiologie des microalgues
Language :
English
Title :
Liver microbiome of Peromyscus leucopus, a key reservoir host species for emerging infectious diseases in North America
Alternative titles :
[fr] le microbiome du foie de Peromyscus leucopus, une espèce réservoir clé pour les maladies infectieuses en Amérique du Nord.
Publication date :
13 April 2017
Journal title :
Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases
ISSN :
1567-1348
eISSN :
1567-7257
Publisher :
Elsevier
Peer reviewed :
Peer Reviewed verified by ORBi
Tags :
CÉCI : Consortium des Équipements de Calcul Intensif
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] NSERC - Natural Sciences and Engineering Research Council [CA]
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