Reference : Purification of DD-carboxypeptidases from Streptomyces strains R61 and K15 by antigen...
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Life sciences : Biochemistry, biophysics & molecular biology
Purification of DD-carboxypeptidases from Streptomyces strains R61 and K15 by antigen-antibody affinity chromatography
Marquet, A []
Nguyen Van-Distèche, Martine mailto [Université de Liège > > Centre d'ingénierie des protéines >]
Leyh-Bouille, Mélina []
Ghuysen, Jean-Marie [Université de Liège - ULiège > Centre d'Ingéniérie des Protéines > > >]
Affinity chromatography : biospecific sorption, the first extensive compendium on affinity chromatography as applied to biochemistry and immunochemistry
Hoffmann-Ostenhof, Otto
[en] purification ; DD ; carboxypeptidases ; streptomyces ; strains ; R61 ; K15 ; antigen ; antibody ; affinity ; chromatography
[en] The exocellular R61 DD-carboxypeptidase and the lysozyme-releasable K15 DD-carboxypeptidase were purified using anti-exocellular R61 enzyme IgG-Sepharose as immunoadsorbent. The specific activity of R61 enzyme was increased 70 fold (yield 90%) and that of K15 enzyme was increased 550 fold (yield 50% of the absorbed material).

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