Reference : Identification of proteins discriminating inflammation induced dysplasia from simple ...
Scientific congresses and symposiums : Unpublished conference/Abstract
Human health sciences : Laboratory medicine & medical technology
Human health sciences : Gastroenterology & hepatology
http://hdl.handle.net/2268/208203
Identification of proteins discriminating inflammation induced dysplasia from simple inflammation in ulcerative colitis by laser capture microdissection and label free proteomics – a pilot study
English
[fr] Identification de protéines discriminant la dysplasie induite par l'inflammation de l'inflammation simple rencontrée dans la rectocolite ulcéro-hémorragique par analyse protéomique sans marquage d'échantillons obtenus par microdissection laser - étude pilote
Merli, Angela-Maria mailto [Université de Liège > Département des sciences cliniques > Hépato-gastroentérologie >]
QUESADA-CALVO, Florence mailto [Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.) >]
MASSOT, Charlotte mailto [Centre Hospitalier Universitaire de Liège - CHU > > Centre d'oncologie >]
BLETARD, Noëlla mailto [Centre Hospitalier Universitaire de Liège - CHU > > Service d'anatomie et cytologie pathologiques >]
Smargiasso, Nicolas mailto [Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.) >]
Baiwir, Dominique mailto [Université de Liège > > GIGA-Technology platforms : Plate-forme protéomique >]
Mazzucchelli, Gabriel mailto [Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.) >]
De Pauw-Gillet, Marie-Claire mailto [Université de Liège > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]
MALAISE, Michel mailto [Centre Hospitalier Universitaire de Liège - CHU > > Service de rhumatologie >]
De Pauw, Edwin mailto [Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.) >]
DELVENNE, Philippe mailto [Centre Hospitalier Universitaire de Liège - CHU > > Service d'anatomie et cytologie pathologiques >]
Meuwis, Marie-Alice mailto [Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.) >]
Louis, Edouard mailto [Université de Liège > Département des sciences cliniques > Hépato-gastroentérologie >]
9-Feb-2017
11
Yes
No
XXIXth edition of the Belgian Week of Gastroenterology
du 9 février 2017 au 11 février 2017
Sven FRANCQUE
Isabelle COLLE
Vlaamse Vereniging voor Gastroenterologie
Société Royale Belge de Gastroentérologie
Belgian Society of Gastrointestinal Endoscopy
Royal Belgian Society of Surgery
Belgian Society of Radiology, Section of Abdominal Imaging
Belgian Association for the Study of the Liver
Belgian Group of Digestive Oncology
Belgian IBD Research Club (BIRD)
Anvers
Belgique
[en] Inflammatory Bowel Diseases ; Ulcerative Colitis ; Dysplasia ; Laser Capture Microdissection ; Label-free Proteomics ; SLC12A2
[en] Chronic colonic inflammation in ulcerative colitis (UC) may induce dysplasia, which can itself progress and transform into neoplasia. Diagnosis of dysplasia in UC remains difficult particularly when tissue inflammation is present.
The aim of this retrospective pilot study was to highlight proteins specifically associated with inflammation induced dysplasia in UC. We performed a pilot experiment on 15 Formalin-Fixed, Paraffin-Embedded (FFPE) samples isolated from 5 cases of UC patients with a Polypoïd Pedunculated dysplasia (UC-PP). We compared the proteomes of the UC-PP, the inflammatory (UC-I) and the normal (UC-NL) tissues of each patient. We performed Laser Capture Microdissection (LCM) in order to collect only epithelial cells, avoiding inflammatory infiltrating ones. Label free proteomic analysis using a 2D-nanoUPLC coupled with a hybrid Quadrupole-Orbitrap was applied, as well as differential analysis on the paired samples. Immunohistochemistry (IHC) characterisation of one of the selected proteins of interest was used for validation.
Out of 985 quantified proteins, 7 were found significantly more abundant in UC-PP compared to UC-I tissues, with 6 being only detected in UC-PP using proteomics. One of these is Solute Carrier Family 12 member 2 (SLC12A2), also known as Na-K-2Cl co-transporter 1 (NKCC1), a protein involved in ionic balance, in T-cell migration promotion and in some features involved in cancer development like proliferation, migration or invasion. IHC results obtained were in correlation with proteomic results and showed that SLC12A2 was more abundant in UC-PP tissue than in UC-I and UC-NL tissues, with a signal clearly delimiting the dysplastic region from the surrounding inflammatory tissue.
This pilot experiment shows a different proteomic profile in inflammation-associated dysplasia and simple inflammation. This should be replicated using other types of dysplasia in IBD. SLC12A2 could be a potential biomarker of inflammation-associated dysplasia.
Giga-Infection, Immunity and Inflammation - Translationnal Gastroenterology
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS - Télévie ; MSD ; Plan Cancer ; SRBGE
Biomarqueurs diagnostiques des dysplasies colorectales (Biomarkers of Colorectal Dysplasia)
Researchers ; Professionals
http://hdl.handle.net/2268/208203

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