Quality of mixing in a stirred bioreactor used for animal cells culture: heterogeneities in a lab scale bioreactor and evolution of mixing time with scale up
[en] Animal cells are industrially cultivated inside stirred bioreactors to produce proteinic compounds. Due to the use of mild agitation conditions in order to limit mechanical constraints, the homogeneity of the culture medium can be far from perfect. This study has therefore two objectives. 1) the global characterization of the mixing via the mixing time 2) the local description of concentration fields. The mixing time is measured by conductimetry inside 20 L, 80 L, 600L tanks. The Grenville correlation is adjusted on these experimental measurements to improve the prediction of the mixing time during the scale-up of the process. The concentration fields are visualized by the Planar Laser Induced Fluorescence (P.L.I.F) technique in the 20 L tank. This part of the study is focused on the time evolution of the maximum value of the tracer concentration inside measurement planes and of the numerical distribution of theses concentration fields.
Research Center/Unit :
Laboratoire de Génie Chimique
Disciplines :
Chemical engineering
Author, co-author :
Collignon, Marie-Laure ; Université de Liège - ULiège > Département de chimie appliquée > Génie chimique - Opérations physiques unitaires
Dossin, Denis; Université de Liège - ULiège > Département de Chimie Appliquée > Laboratoire de Génie Chimique > 2007-2008
Delafosse, Angélique ; Université de Liège - ULiège > Département de chimie appliquée > Génie chimique - Opérations physiques unitaires
Crine, Michel ; Université de Liège - ULiège > Département de chimie appliquée > Génie chimique - Opérations physiques unitaires
Toye, Dominique ; Université de Liège - ULiège > Département de chimie appliquée > Génie de la réaction et des réacteurs chimiques
Language :
English
Title :
Quality of mixing in a stirred bioreactor used for animal cells culture: heterogeneities in a lab scale bioreactor and evolution of mixing time with scale up
Alternative titles :
[en] Qualité du mélange dans un bioréacteur à cuve agitée utilisé pour la culture de cellules animales: étude de l’hétérogénéité à l’échelle laboratoire et prédiction de l’évolution du temps de mélange avec le scale-up
Publication date :
2010
Journal title :
Biotechnologie, Agronomie, Société et Environnement
ISSN :
1370-6233
eISSN :
1780-4507
Publisher :
Presses Agronomiques de Gembloux, Gembloux, Belgium
Volume :
14
Pages :
401-407
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Développement d'un modèle hydrodynamique multi-échelle décrivant le mélange dans les bioréacteurs utilisés en culture cellulaire
Chisti Y., 2000. Animal-cell damage in sparged bioreactors. Trends Biotechnol., 18, 420-432.
Cooke M., Middleton J.C. & Bush J.R., 1988. Mixing and mass transfer in filamentous fermentations. In: King R., ed. Proceedings of the 2nd International conference on bioreactor fluid dynamics, Crandfield, U.K. Cranfield, UK: BHR Group, 37-64.
Grenville R.K., Ruszkowski S. & Garret E., 1995. Blending of miscible liquids in the turbulent and transitional regimes. In: Proceedings of the 15th NAMF mixing conference, Banff, Canada.
Guillard F., Trägardh C. & Fuchs L., 2000. A study of turbulent mixing in a turbine-agitated tank using a fluorescence technique. Exp. Fluids, 28, 225-235.
Houcine I. et al., 1996. Planar laser induced fluorescence technique for measurements of concentration fields in continuous stirred tank reactors. Exp. Fluids, 22, 95-102.
Papoutsakis E.T.,1991.Fluid-mechanicaldamageofanimal- cells in bioreactors. Trends Biotechnol., 9, 427-437.
Ruzskowski S., 1994. A rational method for measurement blending performance and comparison of different impeller types. In: Proceedings of the 8th European mixing conference, Institution of Chemical Engineers, Rugby, U.K., 283-291.
