Reference : Estrogen receptors and estetroldependent neuroprotective actions: a pilot study
Scientific journals : Article
Human health sciences : Neurology
Human health sciences : Reproductive medicine (gynecology, andrology, obstetrics)
Human health sciences : Pediatrics
http://hdl.handle.net/2268/203515
Estrogen receptors and estetroldependent neuroprotective actions: a pilot study
English
Tskitishvili, Ekaterine mailto [Université de Liège > > GIGA-Research >]
Pequeux, Christel mailto [Université de Liège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Munaut, Carine mailto [Université de Liège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
VIELLEVOYE, Renaud mailto [Centre Hospitalier Universitaire de Liège - CHU > > Service néonatologie (CHR) >]
Nisolle, Michelle mailto [Université de Liège > Département des sciences cliniques > Gynécologie - Obstétrique >]
Noël, Agnès mailto [Université de Liège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Foidart, Jean-Michel mailto [Université de Liège > Département des sciences cliniques > Département des sciences cliniques >]
2017
Journal of Endocrinology
Society for Endocrinology
232
1
85-95
Yes (verified by ORBi)
International
0022-0795
1479-6805
Bristol
United Kingdom
[en] hypoxic–ischemic encephalopathy ; estetrol ; ERα ; ERβ ; myelination
[en] Estetrol (E4) has strong antioxidative, neurogenic and angiogenic effects in neural system resulting in the attenuation of neonatal hypoxic-ischemic encephalopathy. We aimed to define the role of estrogen receptors in E4-dependent actions in neuronal cell cultures and prove the promyelinating effect of E4. In vitro the antioxidative and cell survival/proliferating effects of E4 on H2O2-induced oxidative stress in primary hippocampal cell cultures were studied using different combinations of specific inhibitors for ERalpha (MPP dihydrochloride), ERbeta (PHTTP), GPR30 (G15) and palmytoilation (2-BR). LDH activity and cell survival assays were performed. In vivo the promyelinating role of different concentrations of E4 (1 mg/kg/day, 5 mg/kg/day, 10 mg/kg/day, 50 mg/kg/day) was investigated using the hypoxic-ischemic brain damage model in the 7-day-old immature rats before/after the induction of hypoxic-ischemic insult. Myelin basic protein (MBP) immunostaining was performed on brain coronal sections. Our results show that LDH activity is significantly upregulated in cell cultures where the E4's effect was completely blocked by concomitant treatment either with ERalpha and ERbeta inhibitors (MPP and PHTPP, respectively), or ERalpha and ERbeta inhibitors combined with 2-BR. Cell survival is significantly downregulated in cell cultures where the effect of E4 was blocked by ERbeta inhibitor (PHTTP) alone. The blockage of GRP30 receptor did affect neither LDH activity nor cell survival. MBP immunostaining is significantly upregulated in E4-pretreated groups at a concentration of 5 mg/kg/day and 50 mg/kg/day E4, whereas the MBP-positive area OD ratio is significantly increased in all the E4-treated groups. E4's antioxidative actions mostly depend on ERalpha and ERbeta, whereas neurogenesis and possibly promyelinating activities might be realized through ERbeta.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/203515
also: http://hdl.handle.net/2268/203516 ; http://hdl.handle.net/2268/206127
10.1530/JOE-16-0434
(c) 2017 The authors.

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