Article (Scientific journals)
Protein N-glycosylation and N-glycan trimming are required for postembryonic development of the pest beetle Tribolium castaneum
Walski, Tomasz; Van Damme, Els; Smargiasso, Nicolas et al.
2016In Scientific Reports
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Abstract :
[en] In holometabolous insects the transition from larva to adult requires a complete body reorganization and relies on N-glycosylated proteins. N-glycosylation is an important posttranslational modification that influences protein activity but its impact on the metamorphosis has not been studied yet. Here we used the red flour beetle, Tribolium castaneum, to perform a first comprehensive study on the involvement of the protein N-glycosylation pathway in metamorphosis. The transcript levels for genes encoding N-glycan processing enzymes increased during later developmental stages and, in turn, transition from larva to adult coincided with an enrichment of more extensively modified paucimannose glycans, including fucosylated ones. Blockage of N-glycan attachment resulted in larval mortality, while RNAi of α-glucosidases involved in early N-glycan trimming and quality control disrupted the larva to pupa transition. Additionally, simultaneous knockdown of multiple genes responsible for N-glycan processing towards paucimannose structures revealed their novel roles in pupal appendage formation and adult eclosion. Our findings revealed that, next to hormonal control, insect post-embryonic development and metamorphosis depend on protein N-glycan attachment and efficient N-glycan processing. Consequently, disruption of these processes could be an effective new approach for insect control.
Disciplines :
Entomology & pest control
Author, co-author :
Walski, Tomasz;  Universiteit Gent - Ugent > Department of Crop Protection > Laboratory of Agrozoology
Van Damme, Els;  Universiteit Gent - Ugent > Department of Molecular Biotechnology > Laboratory of Biochemistry and Glycobiology
Smargiasso, Nicolas ;  Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Christiaens, Olivier;  Universiteit Gent - Ugent > Department of Crop Protection > Laboratory of Agrozoology
De Pauw, Edwin  ;  Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Smagghe, Guy;  Universiteit Gent - Ugent > Department of Crop Protection > Laboratory of Agrozoology
Language :
English
Title :
Protein N-glycosylation and N-glycan trimming are required for postembryonic development of the pest beetle Tribolium castaneum
Publication date :
2016
Journal title :
Scientific Reports
eISSN :
2045-2322
Publisher :
Nature Publishing Group, London, United Kingdom
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 November 2016

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