[en] Bifidobacterium crudilactis FR62/b/3 belongs to a new population of bifidobacteria isolated from
raw milk and raw milk cheese. The objective of this work was to study the large scale culture of the
stain and its stability in a dry formulation. Growth rate of Bifidobacterium crudilactis FR62/b/3 was
optimal at a pH of 5.0 and a temperature of 37˚C. At a temperature growth of 33˚C and a pH of 5.0,
the stationary phase was reached after 22 h, the viable cell number and the mean dry biomass
concentration were respectively of 8.3 × 109 CFU/mL and of 2.1 g/L. Resistance of Bifidobacterium
crudilactis FR62/b/3 to freeze-drying and effect of a variety of cryoprotectants to maintain the
viability were also evaluated. Sorbitol was the most suitable cryoprotectant for freeze-drying as
well as storage whereas sucrose and monosodium glutamate were only efficient during storage.
Disciplines :
Microbiology
Author, co-author :
Tanimomo, Jean
Delcenserie, Véronique ; Université de Liège > Département de sciences des denrées alimentaires (DDA) > Gestion de la qualité dans la chaîne alimentaire
Taminiau, Bernard ; Université de Liège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Daube, Georges ; Université de Liège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Saint-Hubert, Catherine
Durieux, Alain
Language :
English
Title :
Growth and Freeze-Drying Optimization of Bifidobacterium crudilactis
Publication date :
2016
Journal title :
Food and Nutrition Sciences
ISSN :
2157-944X
eISSN :
2157-9458
Publisher :
Scientific Research, Irvine, United States - California
