[en] Isoflavones are natural plant substances that attract widespread interest because of their association with a large variety of beneficial effects on human health. When they are ingested by animals or humans, most of these compounds are metabolized by bacteria present in the digestive system. Among the isoflavone microbial metabolites, equol is probably the most widely studied because it seems to have numerous health benefits. In the human population, however, not everyone is able to produce equol. Equol enrichment of some food, such as cow's milk, could be an alternative strategy for making equol’s health benefits available to everyone.
The main objective of the present thesis was to develop analytical tools for quantifying these compounds in several biological matrices, thus contributing to the feasibility study being conducted on producing milk naturally enriched with equol. For this purpose, four analytical procedures, using ultra performance liquid chromatography coupled with tandem mass spectrometry (UPLC®-MS/MS), were developed and fully validated. The analytical tools were then applied successfully in two original exploratory studies that sought to quantify equol concentrations in commercial cow's milk in Belgium and study the evolution of isoflavone concentration in red clover (Trifolium pratense L.) silage in laboratory-scale silos.
This thesis describes new and reliable analytical tools that will help to improve current knowledge about the feasibility of producing cow's milk naturally enriched with equol and to study isoflavone metabolization in dairy cow.
Development and validation of analytical methods for quantifying isoflavones and equol in biological matrices
Alternative titles :
[fr] Développement et validation de méthodes analytiques pour la quantification des isoflavones et de l’équol dans des matrices biologiques
Defense date :
19 September 2016
Number of pages :
254
Institution :
ULiège - Université de Liège
Degree :
Docteur en Sciences Agronomiques et Ingenierie Biologique
Promotor :
Lognay, Georges ; Université de Liège - ULiège > Département des sciences et gestion de l'environnement (Arlon Campus Environnement) > Surveillance de l'environnement
Romnee, Jean-Michel
President :
Sindic, Marianne ; Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Jury member :
Froidmont, Eric
Scippo, Marie-Louise ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Beckers, Yves ; Université de Liège - ULiège > Département GxABT > Ingénierie des productions animales et nutrition
Heuskin, Stéphanie ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Analyse, qualité et risques - Laboratoire de Chimie analytique
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.