Doctoral thesis (Dissertations and theses)
Development and validation of analytical methods for quantifying isoflavones and equol in biological matrices
Daems, Frédéric
2016
 

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Keywords :
Isoflavones; Equol; UPLC-MS/MS; Analytical procedure; Quantification; Development; Cow; Silage; Red clover
Abstract :
[en] Isoflavones are natural plant substances that attract widespread interest because of their association with a large variety of beneficial effects on human health. When they are ingested by animals or humans, most of these compounds are metabolized by bacteria present in the digestive system. Among the isoflavone microbial metabolites, equol is probably the most widely studied because it seems to have numerous health benefits. In the human population, however, not everyone is able to produce equol. Equol enrichment of some food, such as cow's milk, could be an alternative strategy for making equol’s health benefits available to everyone. The main objective of the present thesis was to develop analytical tools for quantifying these compounds in several biological matrices, thus contributing to the feasibility study being conducted on producing milk naturally enriched with equol. For this purpose, four analytical procedures, using ultra performance liquid chromatography coupled with tandem mass spectrometry (UPLC®-MS/MS), were developed and fully validated. The analytical tools were then applied successfully in two original exploratory studies that sought to quantify equol concentrations in commercial cow's milk in Belgium and study the evolution of isoflavone concentration in red clover (Trifolium pratense L.) silage in laboratory-scale silos. This thesis describes new and reliable analytical tools that will help to improve current knowledge about the feasibility of producing cow's milk naturally enriched with equol and to study isoflavone metabolization in dairy cow.
Research center :
Centre Wallon de recherches Agronomiques
Disciplines :
Chemistry
Author, co-author :
Daems, Frédéric ;  Université de Liège - ULiège > Doct. sc. agro. & ingé. biol.
Language :
English
Title :
Development and validation of analytical methods for quantifying isoflavones and equol in biological matrices
Alternative titles :
[fr] Développement et validation de méthodes analytiques pour la quantification des isoflavones et de l’équol dans des matrices biologiques
Defense date :
19 September 2016
Number of pages :
254
Institution :
ULiège - Université de Liège
Degree :
Docteur en Sciences Agronomiques et Ingenierie Biologique
Promotor :
Lognay, Georges ;  Université de Liège - ULiège > Département des sciences et gestion de l'environnement (Arlon Campus Environnement) > Surveillance de l'environnement
Romnee, Jean-Michel
President :
Sindic, Marianne  ;  Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
Jury member :
Froidmont, Eric
Scippo, Marie-Louise  ;  Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Beckers, Yves  ;  Université de Liège - ULiège > Département GxABT > Ingénierie des productions animales et nutrition
Heuskin, Stéphanie ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Analyse, qualité et risques - Laboratoire de Chimie analytique
Name of the research project :
PhytoHealth
Funders :
Moerman funds
Available on ORBi :
since 14 September 2016

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